Recombinant Human JAG1 cell lysate

Cat.No. : JAG1-2382HCL
Product Overview : Human JAG1 / JAGL1 / CD339 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : JAG1 jagged 1 [ Homo sapiens ]
Official Symbol : JAG1
Synonyms : JAG1; jagged 1; AGS, Alagille syndrome , JAGL1; protein jagged-1; AHD; AWS; CD339; HJ1; AGS; JAGL1; MGC104644;
Gene ID : 182
mRNA Refseq : NM_000214
Protein Refseq : NP_000205
UniProt ID : P78504
Chromosome Location : 20p12.1-p11.23
Pathway : Activated NOTCH1 Transmits Signal to the Nucleus, organism-specific biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; Notch signaling pathway, organism-specific biosystem; Notch signaling pathway, organism-specific biosystem; Notch signaling pathway, conserved biosystem; Signal Transduction, organism-specific biosystem; Signaling by NOTCH, organism-specific biosystem;
Function : Notch binding; calcium ion binding; growth factor activity; structural molecule activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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06/24/2021

    When used in Western blotting experiments, the JAG1 protein produces distinct protein bands, allowing for easy visualization and interpretation.

    08/29/2020

      Its resistance to degradation and denaturation allows for longer storage times and repeated use without compromising its performance or functionality.

      04/04/2016

        JAG1 protein is known for its stability and robustness, making it suitable for a wide range of experimental conditions.

        Q&As (5)

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        How might knowledge of JAG1 protein be applied in the field of regenerative medicine? 12/26/2020

        Understanding the role of JAG1 in tissue regeneration and repair may lead to advances in regenerative medicine by promoting controlled cell differentiation.

        Are there any clinical tests available for detecting JAG1 mutations in patients? 09/10/2019

        Yes, genetic testing can be performed to identify JAG1 mutations in individuals suspected of having Alagille syndrome or related conditions.

        Are there any targeted therapies or treatments related to the JAG1 protein for Alagille syndrome? 11/06/2017

        Currently, there are no specific targeted therapies for JAG1-related conditions. Treatment typically focuses on managing symptoms and complications.

        Are there any clinical trials currently investigating JAG1-related therapies or treatments? 09/05/2017

        Some clinical trials may be exploring therapies or treatments related to JAG1 in the context of specific medical conditions, but it's essential to check for the most up-to-date information.

        Can JAG1 be a target for cancer therapy? 05/09/2016

        Yes, JAG1 is being investigated as a potential target for cancer therapy, as its role in the Notch pathway can influence cell proliferation and tumor growth.

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