Recombinant Human KITLG Protein, His & Avi-tagged, Biotinylated

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Recombinant Human KITLG Protein, His & Avi-tagged, Biotinylated

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Cat.No. : KITLG-325H
Product Overview : Recombinant Human KITLG Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Glu 26 - Ala 190 (Accession # AAH69797). Predicted N-terminus: Glu 26. This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 21.7 kDa. The protein migrates as 26-38 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Source : HEK293
Species : Human
Tag : His & Avi
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Human CD117, Fc Tag at 2 μg/mL (100 μL/well) can bind Recombinant Human KITLG Protein, His & Avi-tagged, Biotinylated with a linear range of 0.1-3.1 ng/mL (QC tested).
Molecular Mass : 21.7 kDa
Protein length : Glu 26 - Ala 190
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Notes : Please avoid repeated freeze-thaw cycles.
Stability : No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Figure :

Gene Name : KITLG KIT ligand [ Homo sapiens ]
Official Symbol : KITLG
Synonyms : KITLG; KIT ligand; MGF; kit ligand; FPH2; Kitl; KL 1; SCF; SF; steel factor; KL-1; SHEP7; kit-ligand; DKFZp686F2250;
Gene ID : 4254
mRNA Refseq : NM_000899
Protein Refseq : NP_000890
MIM : 184745
UniProt ID : P21583
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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