Recombinant Human KRT16 293 Cell Lysate

• Cat.No.: KRT16-4878HCL

Specification

• Description: Antigen standard for keratin 16 (KRT16) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: KRT16 keratin 16 [ Homo sapiens ]
• Official Symbol: KRT16
• Synonyms: KRT16; keratin 16; keratin, type I cytoskeletal 16; CK-16; keratin-16; cytokeratin 16; cytokeratin-16; focal non-epidermolytic palmoplantar keratoderma; K16; CK16; K1CP; NEPPK; FNEPPK; KRT16A
• Gene ID: 3868
• mRNA Refseq: NM_005557
• Protein Refseq: NP_005548
• Chromosome Location: 17q21.2
• Function: protein binding; structural constituent of cytoskeleton

Reviews

06/08/2022

broad applicability make it a valuable asset for a wide range of research endeavors.

02/20/2019

Researchers can rely on the KRT16 Protein to deliver reliable and reproducible results, facilitating robust data interpretation and scientific advancements.

07/25/2017

It has been widely employed in protein electron microscopy structure analysis, where it has demonstrated remarkable efficacy.

Q&As

Are there any genetic mutations associated with KRT16 protein?

Yes, mutations in the KRT16 gene can lead to various dermatological conditions, such as focal palmoplantar keratoderma.

How is KRT16 protein involved in the pathogenesis of psoriasis?

KRT16 is overexpressed in psoriatic skin lesions and plays a role in the abnormal keratinocyte proliferation and differentiation seen in psoriasis.

Can KRT16 protein be used in regenerative medicine applications?

There is growing interest in utilizing KRT16 protein in tissue engineering and regenerative medicine approaches for skin and wound healing.

How is KRT16 protein used in the diagnosis of skin disorders?

KRT16 protein expression can be assessed through immunohistochemistry or molecular techniques to aid in the diagnosis and classification of skin disorders.

How do changes in KRT16 protein expression affect the skin barrier function?

Alterations in KRT16 expression can impact the integrity and function of the skin barrier, leading to increased susceptibility to environmental insults and potential skin disorders.