Recombinant Human KRT16 protein, His-tagged KRT16-2720H

Recombinant Human KRT16 protein, His-tagged

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Recombinant Human KRT16 protein, His-tagged

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Cat.No. : KRT16-2720H
Product Overview : Recombinant Human KRT16 aa. (Glu117~Arg419) fused with N-terminal His tag was produced in E. coli cells.
Description : The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus.
Source : E. coli
Species : Human
Tag : His
Form : Freeze-dried powder
Molecular Mass : 39kDa as determined by SDS-PAGE reducing conditions.
Protein length : Glu117~Arg419
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >98%
Characteristic : The isoelectric point is 4.9.
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200μg/mL
Storage buffer : 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
Reconstitution : Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex
SDS-PAGE :

Gene Name : KRT16 keratin 16 [ Homo sapiens (human) ]
Official Symbol : KRT16
Synonyms : K16; PC1; CK16; K1CP; NEPPK; FNEPPK; KRT16A; cytokeratin 16; focal non-epidermolytic palmoplantar keratoderma; keratin 16, type I; keratin, type I cytoskeletal 16
Gene ID : 3868
mRNA Refseq : NM_005557.3
Protein Refseq : NP_005548.2
UniProt ID : P08779

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