Active Recombinant Human Leptin Protein, Tag Free
Cat.No. : | LEP-3626H |
Product Overview : | Recombinant Human LEP protein (NP_000221.1) (Val 22-Cys 167) was expressed in E.coli, with an additional Met. |
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Species : | Human |
Source : | E.coli |
Tag : | Non |
Protein Length : | 22-167 aa |
Form : | Lyophilized from sterile PBS, pH 7.4. |
Bio-activity : | Immobilized Human Leptin at 2 μg/mL (100 μL/well) can bind Human Leptin Receptor (His & hFc Tag), the EC50 of Human Leptin Receptor (His & hFc Tag) is 70-420 ng/mL. |
Molecular Mass : | The recombinant human leptin consists of 147 amino acids and predicts a molecular mass of 16 kDa. The apparent molecular mass of rhLEP is approximately 13 kDa in SDS-PAGE under reducing conditions. |
Purity : | > 98 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.13mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name | LEP leptin [ Homo sapiens ] |
Official Symbol | LEP |
Synonyms | LEP; leptin; leptin (murine obesity homolog) , leptin (obesity homolog, mouse) , OB, OBS; obese protein; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse); OB; OBS; FLJ94114; |
Gene ID | 3952 |
mRNA Refseq | NM_000230 |
Protein Refseq | NP_000221 |
UniProt ID | P41159 |
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◆ Native Proteins | ||
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◆ Cell & Tissue Lysates | ||
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Not For Human Consumption!
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