Recombinant Human LIG3 Protein, His-tagged

• Cat.No.: LIG3-68H
• Product Overview: Recombinant Human LIG3 Protein, fused to His-tag, was expressed in E. coli.

Specification

• Description: This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Supplied as a 0.2 μm filtered solution in PBS, pH 8.0.
• Molecular Mass: ~103.6 kDa
• AA Sequence: MHHHHHHMAEQRFCVDYAKRGTAGC KKCKEKIVKGVCRIGKVVPNPFSES GGDMKEWYHIKCMFEKLERARATTK KIEDLTELEGWEELEDNEKEQITQH IADLSSKAAGTPKKKAVVQAKLTTT GQVTSPVKGASFVTSTNPRKFSGFS AKPNNSGEAPSSPTPKRSLSSSKCD PRHKDCLLREFRKLCAMVADNPSYN TKTQIIQDFLRKGSAGDGFHGDVYL TVKLLLPGVIKTVYNLNDKQIVKLF SRIFNCNPDDMARDLEQGDVSETIR VFFEQSKSFPPAAKSLLTIQEVDEF LLRLSKLTKEDEQQQALQDIASRCT ANDLKCIIRLIKHDLKMNSGAKHVL DALDPNAYEAFKASRNLQDVVERVL HNAQEVEKEPGQRRALSVQASLMTP VQPMLAEACKSVEYAMKKCPNGMFS EIKYDGERVQVHKNGDHFSYFSRSL KPVLPHKVAHFKDYIPQAFPGGHSM ILDSEVLLIDNKTGKPLPFGTLGVH KKAAFQDANVCLFVFDCIYFNDVSL MDRPLCERRKFLHDNMVEIPNRIMF SEMKRVTKALDLADMITRVIQEGLE GLVLKDVKGTYEPGKRHWLKVKKDY LNEGAMADTADLVVLGAFYGQGSKG GMMSIFLMGCYDPGSQKWCTVTKCA GGHDDATLARLQNELDMVKISKDPS KIPSWLKVNKIYYPDFIVPDPKKAA VWEITGAEFSKSEAHTADGISIRFP RCTRIRDDKDWKSATNLPQLKELYQ LSKEKADFTVVAGDEGSSTTGGSSE ENKGPSGSAVSRKAPSKPSASTKKA EGKLSNSNSKDGNMQTAKPSAMKVG EKLATKSSPVKVGEKRKAADETLCQ TKVLLDIFTGVRLYLPPSTPDFSRL RRYFVAFDGDLVQEFDMTSATHVLG SRDKNPAAQQVSPEWIWACIRKRRL VAPC
• Purity: >85%
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.11mg/mL

Gene Information

• Gene Name: LIG3 DNA ligase 3 [ Homo sapiens (human) ]
• Official Symbol: LIG3
• Synonyms: LIG2; MTDPS20; LIG3alpha
• Gene ID: 3980
• mRNA Refseq: NM_002311
• Protein Refseq: NP_002302
• MIM: 600940
• UniProt ID: P49916