Recombinant Full Length Human LIG3 Protein, C-Flag-tagged

• Cat.No.: LIG3-599HFL
• Product Overview: Recombinant Full Length Human LIG3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 108.6 kDa
• AA Sequence: MSLAFKIFFPQTLRALSRKELCLFR KHHWRDVRQFSQWSETDLLHGHPLF LRRKPVLSFQGSHLRSRATY LVFLPGLHVGLCSGPCEMAEQRFCV DYAKRGTAGCKKCKEKIVKGVCRIG KVVPNPFSESGGDMKEWYHI KCMFEKLERARATTKKIEDLTELEG WEELEDNEKEQITQHIADLSSKAAG TPKKKAVVQAKLTTTGQVTS PVKGASFVTSTNPRKFSGFSAKPNN SGEAPSSPTPKRSLSSSKCDPRHKD CLLREFRKLCAMVADNPSYN TKTQIIQDFLRKGSAGDGFHGDVYL TVKLLLPGVIKTVYNLNDKQIVKLF SRIFNCNPDDMARDLEQGDV SETIRVFFEQSKSFPPAAKSLLTIQ EVDEFLLRLSKLTKEDEQQQALQDI ASRCTANDLKCIIRLIKHDL KMNSGAKHVLDALDPNAYEAFKASR NLQDVVERVLHNAQEVEKEPGQRRA LSVQASLMTPVQPMLAEACK SVEYAMKKCPNGMFSEIKYDGERVQ VHKNGDHFSYFSRSLKPVLPHKVAH FKDYIPQAFPGGHSMILDSE VLLIDNKTGKPLPFGTLGVHKKAAF QDANVCLFVFDCIYFNDVSLMDRPL CERRKFLHDNMVEIPNRIMF SEMKRVTKALDLADMITRVIQEGLE GLVLKDVKGTYEPGKRHWLKVKKDY LNEGAMADTADLVVLGAFYG QGSKGGMMSIFLMGCYDPGSQKWCT VTKCAGGHDDATLARLQNELDMVKI SKDPSKIPSWLKVNKIYYPD FIVPDPKKAAVWEITGAEFSKSEAH TADGISIRFPRCTRIRDDKDWKSAT NLPQLKELYQLSKEKADFTV VAGDEGSSTTGGSSEENKGPSGSAV SRKAPSKPSASTKKAEGKLSNSNSK DGNMQTAKPSAMKVGEKLAT KSSPVKVGEKRKAADETLCQTKVLL DIFTGVRLYLPPSTPDFSRLRRYFV AFDGDLVQEFDMTSATHVLG
  SRDKNPAAQQVSPEWIWACIRKRRL VAPCTRTRPLEQKLISEEDLAANDI LDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair

Gene Information

• Gene Name: LIG3 DNA ligase 3 [ Homo sapiens (human) ]
• Official Symbol: LIG3
• Synonyms: LIG2; MTDPS20; LIG3alpha
• Gene ID: 3980
• mRNA Refseq: NM_013975.4
• Protein Refseq: NP_039269.2
• MIM: 600940
• UniProt ID: P49916