Recombinant Human Mdm2 p53 Binding Protein Homolog, GST-tagged

• Cat.No.: MDM2-1063H
• Product Overview: Recombinant human MDM2 (202-end) was expressed in E. coli cells using an N-terminal GST tag.

Specification

• Cat. No.: MDM2-1063H
• Description: MDM2 is a nuclear phosphoprotein that binds and inhibits transactivation by p53, as part of an autoregulatory negative feedback loop (1). Overexpression of the MDM2 gene product can lead to excessive inactivation of p53 thereby diminishing its tumor suppressor function. The inactivation of p53 is mediated by the E3 ubiquitin ligase activity of MDM2 which targets p53 for proteasomal degradation. MDM2 also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5 (2). Amplification of MDM2 is frequently observed in human sarcomas and this is consistent with the hypothesis that MDM2 binds to p53 which then leads to escape from p53-regulated growth control.
• Source: Human
• Host: E. coli
• Applications: Western Blot
• Molecular Weight: ~84 kDa
• Purity: >75%
• Concentration: 0.2ug/ul
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25%glycerol.
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: AKT phosphorylates targets in the cytosol; Bladder cancer; Androgen Receptor Signaling Pathway; Cell Cycle Checkpoints; Apoptosis; Cell cycle; Aurora A signaling; Chronic myeloid leukemia; Direct p53 effectors; Endocytosis; Downstream signaling of activated FGFR; ErbB signaling pathway; G1 to S cell cycle control; G1/S DNA Damage Checkpoints; Glioma; Glucocorticoid receptor regulatory network; Glutamate Binding, Activation of AMPA Receptors and Synaptic Plasticity; Melanoma; NGF signalling via TRKA from the plasma membrane; Neuroransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell; PI-3K cascade; PI3K/AKT activation; Signaling by FGFR; PIP3 activates AKT signaling; Regulation of retinoblastoma protein; Sumoylation by RanBP2 regulates transcriptional repression

Gene Information

• Gene Name: MDM2 Mdm2 p53 binding protein homolog (mouse) [ Homo sapiens ]
• Official Symbol: MDM2
• Synonyms: MDM2; Mdm2 p53 binding protein homolog (mouse); HDMX; hdm2; MGC5370; MGC71221; E3 ubiquitin-protein ligase Mdm2; oncoprotein Mdm2; MDM2 variant FB28; MDM2 variant FB30; OTTHUMP00000183488; OTTHUMP00000183489; OTTHUMP00000183495; OTTHUMP00000183496; OTTHUMP00000240280; OTTHUMP00000240285; OTTHUMP00000240290; ubiquitin-protein ligase E3 Mdm2; double minute 2 human homolog of p53-binding protein; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; p53-binding protein Mdm2; Oncoprotein Mdm2; mouse double minute 2, human homolog of; p53-binding protein; ubiquitin-protein ligase E3 Mdm2; EC 6.3.2; Double minute 2 protein
• Gene ID: 4193
• mRNA Refseq: NM_002392
• Protein Refseq: NP_002383
• MIM: 164785
• UniProt ID: Q00987
• Chromosome Location: 12q14.3-q15
• Function: zinc ion binding; ubiquitin-protein ligase activity; protein binding; p53 binding; metal ion binding; ligase activity; enzyme binding

Reviews

12/24/2021

Validated in multiple assays.

07/14/2019

Great for cell culture.

04/05/2019

Consistent performance, highly reproducible.

Q&As

How does MDM2 regulate the tumor suppressor protein p53?

MDM2 binds directly to p53, inhibiting its transcriptional activity. Additionally, MDM2 ubiquitinates p53, targeting it for proteasomal degradation, thus regulating p53 levels in the cell.

What is the significance of the MDM2-p53 feedback loop in cellular regulation?

The MDM2-p53 feedback loop is crucial for cellular homeostasis. When DNA damage occurs, p53 levels rise, leading to cell cycle arrest or apoptosis. Elevated p53 can induce MDM2 expression, which in turn downregulates p53, ensuring that p53 activity is transient.

How do amplifications or overexpression of MDM2 contribute to cancer progression?

Amplifications or overexpression of MDM2 can lead to reduced p53 activity, even in the presence of DNA damage or other stress signals, potentially contributing to uncontrolled cell proliferation and tumorigenesis.

Are there any known inhibitors targeting MDM2, and what are their therapeutic implications?

Several small molecule inhibitors, like Nutlin-3 and RG7112, target MDM2 to prevent its interaction with p53, thereby stabilizing and activating p53. These inhibitors hold therapeutic potential, especially in tumors with wild-type p53 but overexpressed MDM2.

How does the interaction between MDM2 and other proteins influence cell cycle and apoptosis?

Besides p53, MDM2 interacts with other proteins, including E2F1 and RB, influencing cell cycle progression and apoptosis. The balance of these interactions can determine cell fate.

What is the primary function of the MDM2 gene and its encoded protein?

The MDM2 gene encodes a nuclear-localized E3 ubiquitin ligase. Its primary function is to regulate the p53 tumor suppressor protein's stability and activity.

What are the potential therapeutic strategies for targeting MDM2 in cancer treatment?

Therapeutic strategies targeting MDM2 include the use of MDM2 inhibitors to reactivate p53, combination therapies with DNA-damaging agents, and strategies to disrupt MDM2's interaction with other proteins to modulate cell cycle and apoptosis.