Recombinant Human MDM2 Protein (S17-N111), His tagged

• Cat.No.: MDM2-0427H
• Product Overview: Recombinant Human 6His-TEV-MDM2(S17-N111) Protein was expressed in E. coli.

Specification

• Description: E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation. Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells. Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis. Negatively regulates NDUFS1, leading to decreased mitochondrial respiration, marked oxidative stress, and commitment to the mitochondrial pathway of apoptosis. Binds NDUFS1 leading to its cytosolic retention rather than mitochondrial localization resulting in decreased supercomplex assembly (interactions between complex I and complex III), decreased complex I activity, ROS production, and apoptosis.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid
• Protein Length: S17-N111
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50mM Tris-HCl (pH 7.5), 200mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: MDM2 Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) [ Homo sapiens (human) ]
• Official Symbol: MDM2
• Synonyms: MDM2; Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse); Mdm2 p53 binding protein homolog (mouse), Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse), mouse double minute 2, human homolog of; p53 binding protein; E3 ubiquitin-protein ligase Mdm2; HDM2; HDMX; MGC5370; oncoprotein Mdm2; MDM2 variant FB28; MDM2 variant FB30; double minute 2, human homolog of; p53-binding protein; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; hdm2; ACTFS; MGC71221
• Gene ID: 4193
• mRNA Refseq: NM_002392
• Protein Refseq: NP_002383
• MIM: 164785
• UniProt ID: Q00987

Reviews

12/24/2021

Validated in multiple assays.

07/14/2019

Great for cell culture.

04/05/2019

Consistent performance, highly reproducible.

Q&As

How does MDM2 regulate the tumor suppressor protein p53?

MDM2 binds directly to p53, inhibiting its transcriptional activity. Additionally, MDM2 ubiquitinates p53, targeting it for proteasomal degradation, thus regulating p53 levels in the cell.

What is the significance of the MDM2-p53 feedback loop in cellular regulation?

The MDM2-p53 feedback loop is crucial for cellular homeostasis. When DNA damage occurs, p53 levels rise, leading to cell cycle arrest or apoptosis. Elevated p53 can induce MDM2 expression, which in turn downregulates p53, ensuring that p53 activity is transient.

How do amplifications or overexpression of MDM2 contribute to cancer progression?

Amplifications or overexpression of MDM2 can lead to reduced p53 activity, even in the presence of DNA damage or other stress signals, potentially contributing to uncontrolled cell proliferation and tumorigenesis.

Are there any known inhibitors targeting MDM2, and what are their therapeutic implications?

Several small molecule inhibitors, like Nutlin-3 and RG7112, target MDM2 to prevent its interaction with p53, thereby stabilizing and activating p53. These inhibitors hold therapeutic potential, especially in tumors with wild-type p53 but overexpressed MDM2.

How does the interaction between MDM2 and other proteins influence cell cycle and apoptosis?

Besides p53, MDM2 interacts with other proteins, including E2F1 and RB, influencing cell cycle progression and apoptosis. The balance of these interactions can determine cell fate.

What is the primary function of the MDM2 gene and its encoded protein?

The MDM2 gene encodes a nuclear-localized E3 ubiquitin ligase. Its primary function is to regulate the p53 tumor suppressor protein's stability and activity.

What are the potential therapeutic strategies for targeting MDM2 in cancer treatment?

Therapeutic strategies targeting MDM2 include the use of MDM2 inhibitors to reactivate p53, combination therapies with DNA-damaging agents, and strategies to disrupt MDM2's interaction with other proteins to modulate cell cycle and apoptosis.