Recombinant Human MDM2 Protein, His-tagged
Cat.No. : | MDM2-568H |
Product Overview : | Recombinant Human MDM2(Q00987-4)(Met1~Pro218) fused with His tag at N-terminal was expressed in E. coli. |
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Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% skl and 5% trehalose. |
Molecular Mass : | 44kDa as determined by SDS-PAGE reducing conditions. |
Protein length : | Met1~Pro218 |
Endotoxin : | <1.0EU per 1μg (determined by the LAL method). |
Purity : | >95% |
Applications : | Cell culture; Activity Assays. |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80°C for 12 months. |
Reconstitution : | Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex. |
Gene Name : | MDM2 Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) [ Homo sapiens ] |
Official Symbol : | MDM2 |
Synonyms : | MDM2; Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse); Mdm2 p53 binding protein homolog (mouse) , Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse) , mouse double minute 2, human homolog of; p53 binding protein; E3 ubiquitin-protein ligase Mdm2; HDM2; HDMX; MGC5370; oncoprotein Mdm2; MDM2 variant FB28; MDM2 variant FB30; double minute 2, human homolog of; p53-binding protein; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; hdm2; ACTFS; MGC71221; |
Gene ID : | 4193 |
mRNA Refseq : | NM_002392.4 |
Protein Refseq : | NP_002383.2 |
MIM : | 164785 |
UniProt ID : | Q00987 |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionMDM2 binds directly to p53, inhibiting its transcriptional activity. Additionally, MDM2 ubiquitinates p53, targeting it for proteasomal degradation, thus regulating p53 levels in the cell.
The MDM2-p53 feedback loop is crucial for cellular homeostasis. When DNA damage occurs, p53 levels rise, leading to cell cycle arrest or apoptosis. Elevated p53 can induce MDM2 expression, which in turn downregulates p53, ensuring that p53 activity is transient.
Amplifications or overexpression of MDM2 can lead to reduced p53 activity, even in the presence of DNA damage or other stress signals, potentially contributing to uncontrolled cell proliferation and tumorigenesis.
Several small molecule inhibitors, like Nutlin-3 and RG7112, target MDM2 to prevent its interaction with p53, thereby stabilizing and activating p53. These inhibitors hold therapeutic potential, especially in tumors with wild-type p53 but overexpressed MDM2.
Besides p53, MDM2 interacts with other proteins, including E2F1 and RB, influencing cell cycle progression and apoptosis. The balance of these interactions can determine cell fate.
The MDM2 gene encodes a nuclear-localized E3 ubiquitin ligase. Its primary function is to regulate the p53 tumor suppressor protein's stability and activity.
Therapeutic strategies targeting MDM2 include the use of MDM2 inhibitors to reactivate p53, combination therapies with DNA-damaging agents, and strategies to disrupt MDM2's interaction with other proteins to modulate cell cycle and apoptosis.
Customer Reviews (3)
Write a reviewValidated in multiple assays.
Great for cell culture.
Consistent performance, highly reproducible.
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