Recombinant Human MGMT Protein, His-tagged

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Recombinant Human MGMT Protein, His-tagged

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Cat.No. : MGMT-075H
Product Overview : Recombinant active N-terminal His-tagged human MGMT protein was expressed in E. coli.
Description : Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma.
Source : E. coli
Species : Human
Tag : His
Molecular Mass : 26.8 kDa
Purity : ≥50% estimated by SDS-PAGE
Stability : ≥ 6 months
Storage : At -80 centigrade.
Storage Buffer : 50 mM Tris, pH 8.0, 150 mM sodium chloride, 10% glycerol
SDS-PAGE analysis of MGMT :

Lane 1: MW Markers
Lane 2: MGMT (1 μg)
Lane 3: MGMT (2 μg)
Lane 4: MGMT (4 μg)

Gene Name : MGMT O-6-methylguanine-DNA methyltransferase [ Homo sapiens (human) ]
Official Symbol : MGMT
Synonyms : MGMT; O-6-methylguanine-DNA methyltransferase; methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; methylguanine-DNA methyltransferase; EC 2.1.1.63
Gene ID : 4255
mRNA Refseq : NM_002412
Protein Refseq : NP_002403
MIM : 156569
UniProt ID : B4DEE8
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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