Recombinant Human Monocyte Chemoattractant Protein 1

• Cat.No.: CCL2-1188H
• Product Overview: RecombinantHuman CCL2 producedin E. coli is a single, non-glycosylated, polypeptide chain containing 76 aminoacids.

Specification

• Cat. No.: CCL2-1188H
• Description: CCL2is a protein that in humans is encoded by the CCL2 gene. CCL2 is a smallcytokine belonging to the CC chemokine family. CCL2 recruits monocytes,memory T cells, and dendritic cells to sites of tissue injury, infection, andinflammation.
• Source: E. coli
• Species: Human
• Form: Lyophilizedfrom a 0.2um filtered concentrated (1.0 mg/ml) solution in 20 mM PB, pH 7.4 +150 mM NaCl.
• Molecular Weight: 8.6 kDa
• Endotoxin Level: < 0.1 ng/ug (1EU/ug) of protein.
• Purity: >97% as determined by HPLC and SDS-PAGE analyses.
• Biological Activity: Measuredby its ability to chemoattract human monocytes using a concentration range of5 - 20 ng/ml.
• Amino Acid Sequence: QPDAINAPVTCCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT
• Reconstitution: Centrifuge vial prior to opening. Addsterile distilled water or aqueous buffer containing 0.1% BSA to aconcentration of 0.1-1.0 mg/ml. Please note that the addition of any carrierprotein into this product may produce unwanted endotoxin. This depends uponthe particular application employed. Further dilutions should be made inappropriate buffered solutions.
• Storage: Stable at2-8°C, but best kept desiccated -20°C. Upon reconstitution, stable for up to1 week at 2-8°C. For longer term, store in working aliquots below -20°C.Avoid repeated freeze/thaw cycles.
• OfficialSymbol: CCL2

Gene Information

• Gene Name: CCL2chemokine (C-C motif) ligand 2 [ Homo sapiens ]
• Synonyms: CCL2; chemokine(C-C motif) ligand 2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF;HSMCR30; MGC9434; small inducible cytokine A2 (monocyte chemotactic protein1, homologous to mouse Sig-je); monocyte chemoattractant protein-1; monocytechemotactic and activating factor; monocyte chemotactic protein 1; homologousto mouse Sig-je; monocyte secretory protein JE; small inducible cytokinesubfamily A (Cys-Cys), member 2; OTTHUMP00000163799; C-C motif chemokine 2; Monocytechemoattractant protein 1; Small-inducible cytokine A2
• Gene ID: 6347
• mRNA Refseq: NM_002982
• Protein Refseq: NP_002973
• MIM: 158105
• UniProt ID: P13500
• Chromosome Location: 17q11.2-q12
• Pathway: Chagasdisease (American trypanosomiasis); Chemokine receptors bind chemokines; Chemokinesignaling pathway; Class A/1 (Rhodopsin-like receptors); Cytokine-cytokinereceptor interaction; Diabetes pathways; GPCR ligand binding; Herpes simplexinfection; IL23-mediated signaling events; Influenza A; Malaria; NOD-likereceptor signaling pathway; PERK regulated gene expression; Peptideligand-binding receptors; Rheumatoid arthritis
• Function: CCR2 chemokine receptor binding; G-protein couple receptorbinding; chemokine activity; heparin binding; protein kinase activity; receptorbinding; signal transducer activity