Active Recombinant Mouse Chemokine (C-C motif) Ligand 2

• Cat.No.: Ccl2-282M
• Product Overview: Recombinant Murine JE(MCP-1) produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 13803 Dalton.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Description: Chemokine (C-C motif) ligand 2 (CCL2) is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1) and small inducible cytokine A2. CCL2 recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury, infection, and inflammation.
• Form: Lyophilized with no additives.
• Purity: Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Bio-activity: Murine JE(MCP-1)is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract Balb/C mouse spleen MNCs at 1.0-20.0 ng/ml.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mgμs/ml, which can be further diluted into other aqueous solutions.
• Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Protein Content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
• Amino acid sequence: QPDAVNAPLT CCYSFTSKMI PMSRLESYKR ITSSRCPKEA VVFVTKLKRE VCADPKKEW-V QTYIKNLDRN QMRSEPTTLF KTASALRSSA PLNVK-LTRKS EANASTTFST TTSSTSVGVT SVTVN
• Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
• Pathways: Chemokine signaling pathway; Cytokine-cytokine receptor interaction

Gene Information

• Gene Name: Ccl2 chemokine (C-C motif) ligand 2 [ Mus musculus ]
• Official Symbol: Ccl2
• Synonyms: Ccl2; chemokine (C-C motif) ligand 2; JE; HC11; MCAF; MCP1; MCP-1; Scya2; Sigje; SMC-CF; AI323594; monocyte chemoattractant protein-1; monocyte chemotactic protein; OTTMUSP00000000786; small inducible cytokine A2; monocyte chemotactic protein
• Gene ID: 20296
• mRNA Refseq: NM_011333
• Protein Refseq: NP_035463
• UniProt ID: P10148
• Chromosome Location: 11 C-E1; 11 46.5 cM
• Function: G-protein-coupled receptor binding; chemokine activity; cytokine activity; protein binding