Recombinant Human MUC2 protein, His-tagged

• Cat.No.: MUC2-285H
• Product Overview: Recombinant Human MUC2 protein(Cys5185-Gly5289), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 5185-5289 aa
• Form: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Molecular Mass: 12.5kDa
• AASequence: MGHHHHHHSGSEF-CSTVPV TTEVSYAGCT KTVLMNHCSG SCGTFVMYSA KAQALDHSCS CCKEEKTSQR EVVLSCPNGG SLTHTYTHIE SCQCQDTVCG LPTGTSRRAR RSPRHLGSG
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 80%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: MUC2 mucin 2, oligomeric mucus/gel-forming [ Homo sapiens ]
• Official Symbol: MUC2
• Synonyms: MUC2; mucin 2, oligomeric mucus/gel-forming; mucin 2, intestinal/tracheal; mucin-2; intestinal mucin-2; mucin-like protein; MLP; SMUC; MUC-2
• Gene ID: 4583
• mRNA Refseq: NM_002457
• Protein Refseq: NP_002448
• MIM: 158370
• UniProt ID: Q02817

Citation

The Vat-AIEC protease promotes crossing of the intestinal mucus layer by Crohn's disease-associated Escherichia coli.

Journal: Cellular microbiology    PubMed ID: 26499863    Data: 2016/12/29

Authors: Lucie Gibold, Estelle Garenaux, Julien Delmas

Article Snippet:Muc2 proteolytic cleavage assay One-hundred microlitres of culture supernatants filter-sterilized (0.22 μm) or 1 μg of purified fraction of Vat-AIEC were distributed to 1 μg of mucins followed by overnight digestion at 37 °C.Muc2 proteolytic cleavage assay One-hundred microlitres of culture supernatants filter-sterilized (0.22 μm) or 1 μg of purified fraction of Vat-AIEC were distributed to 1 μg of mucins followed by overnight digestion at 37 °C.. Mucins were fractions of extracted mucus from mouse intestine or a fragment of the C-terminal cysteine-rich domain of an unglycosylated recombinant human MUC2, which does not harbour the autocatalytic cleavage site of MUC2 (Creative BioMart, USA). . The digestion was terminated by incubating samples with 4× Laemmli sample buffer containing 10% mercaptoethanol at 100 °C for 3min.The digestion was terminated by incubating samples with 4× Laemmli sample buffer containing 10% mercaptoethanol at 100 °C for 3min.