Recombinant Human MUC2 protein, His-tagged

• Cat.No.: MUC2-285H
• Product Overview: Recombinant Human MUC2 fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
• Form: Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.
• Molecular Mass: 12.7kDa
• AA Sequence: MGHHHHHHSGSEF- CSTVPV TTEVSYAGCT KTVLMNHCSG SCGTFVMYSA KAQALDHSCS CCKEEKTSQR EVVLSCPNGG SLTHTYTHIE SCQCQDTVCG LPTGTSRRAR RSPRHLGSG
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: MUC2 mucin 2, oligomeric mucus/gel-forming [ Homo sapiens ]
• Official Symbol: MUC2
• Synonyms: MUC2; mucin 2, oligomeric mucus/gel-forming; mucin 2, intestinal/tracheal; mucin-2; intestinal mucin-2; mucin-like protein; MLP; SMUC; MUC-2
• Gene ID: 4583
• mRNA Refseq: NM_002457
• Protein Refseq: NP_002448
• MIM: 158370
• UniProt ID: Q02817
• Chromosome Location: 11p15.5
• Pathway: Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Metabolism of proteins, organism-specific biosystem; O-linked glycosylation of mucins, organism-specific biosystem; Post-translational protein modification, organism-specific biosystem; Termination of O-glycan biosynthesis, organism-specific biosystem; Vibrio cholerae infection, organism-specific biosystem
• Function: protein binding

Citation

The Vat-AIEC protease promotes crossing of the intestinal mucus layer by Crohn's disease-associated Escherichia coli.

Journal: Cellular microbiology    PubMed ID: 26499863    Data: 2016/12/29

Authors: Lucie Gibold, Estelle Garenaux, Julien Delmas

Article Snippet:Muc2 proteolytic cleavage assay One-hundred microlitres of culture supernatants filter-sterilized (0.22 μm) or 1 μg of purified fraction of Vat-AIEC were distributed to 1 μg of mucins followed by overnight digestion at 37 °C.Muc2 proteolytic cleavage assay One-hundred microlitres of culture supernatants filter-sterilized (0.22 μm) or 1 μg of purified fraction of Vat-AIEC were distributed to 1 μg of mucins followed by overnight digestion at 37 °C.. Mucins were fractions of extracted mucus from mouse intestine or a fragment of the C-terminal cysteine-rich domain of an unglycosylated recombinant human MUC2, which does not harbour the autocatalytic cleavage site of MUC2 (Creative BioMart, USA). . The digestion was terminated by incubating samples with 4× Laemmli sample buffer containing 10% mercaptoethanol at 100 °C for 3min.The digestion was terminated by incubating samples with 4× Laemmli sample buffer containing 10% mercaptoethanol at 100 °C for 3min.