Recombinant Human PDE1C cell lysate

Cat.No. : PDE1C-001HCL
Product Overview : Human PDE1C derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : PDE1C phosphodiesterase 1C, calmodulin-dependent 70kDa [ Homo sapiens ]
Official Symbol : PDE1C
Synonyms : PDE1C; phosphodiesterase 1C, calmodulin-dependent 70kDa; phosphodiesterase 1C, calmodulin dependent (70kD); calcium/calmodulin-dependent 3,5-cyclic nucleotide phosphodiesterase 1C; Hcam3; hCam-3; cam-PDE 1C; Human 3,5 cyclic nucleotide phosphodiesterase (HSPDE1C1A);
Gene ID : 5137
mRNA Refseq : NM_001191056
Protein Refseq : NP_001177985
MIM : 602987
UniProt ID : Q14123
Chromosome Location : 7p15.1-p14.3
Pathway : Ca-dependent events, organism-specific biosystem; CaM pathway, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Calmodulin induced events, organism-specific biosystem; Cam-PDE 1 activation, organism-specific biosystem; DAG and IP3 signaling, organism-specific biosystem;
Function : calmodulin binding; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity; hydrolase activity; metal ion binding; phosphoric diester hydrolase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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