PDE2A-07H
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant human PDE2A protein (215-900) with a C-terminal His-tag was expressed in insect cells.
Description :
Phosphodiesterase 2A (PDE2A) is a cGMP-stimulated cyclic nucleotide phosphodiesterase that hydrolyzes cGMP and cAMP. It is a 941-amino acid protein comprised of an N-terminal domain (1-214), GAF-A domain (215-372), GAF-B domain (393-541), and a catalytic domain (579-941) and functions as a homodimer. PDE2A is expressed in the brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney, spleen, and pituitary. Upon cGMP binding to the GAF-B domain, PDE2A is activated and hydrolyzes cAMP and cGMP to regulate cell signaling. PDE2A mRNA levels are reduced in postmortem amygdala from patients with schizophrenia, bipolar disorder, and major depressive disorder compared with healthy controls. PDE2A brain activity is increased in the Fmr1-/- mouse model of fragile X syndrome and administration of the selective PDE2A inhibitor BAY 60-7550 restores axonal length and spine maturation and attenuates social discriminatory and vocalization deficits in Fmr1-/- young mice. Adenovirus shRNA knockdown of Pde2aattenuates bronchoalveolar lavage fluid (BALF) neutrophilia, reduces lung levels of inducible nitric oxide synthase (iNOS), and increases lung cAMP levels in a mouse model of acute lung injury.
Source :
Insect cell
Species :
Human
Tag :
His
Form :
Lyophilized from sterile 20mM Tris, pH 7.4, with 500 mM sodium chloride and 10% glycol
Molecular Mass :
80 kDa
Protein length :
215-900
Endotoxin :
<1.0 EU/μg, determined by the LAL endotoxin assay
Purity :
≥90% estimated by SDS-PAGE
Stability :
≥ 1 year
Storage :
Store at -80 centigrade
SDS-PAGE Analysis of PDE2A :
Lane 1: MW Markers, Lane 2: PDE2A(human, recombinant; aa215-900)
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.