Recombinant Human PDE2A cell lysate

• Cat.No.: PDE2A-591HCL
• Product Overview: Human PDE2A / CGS-PDE (aa 215 - 900 ) derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Baculovirus-Insect Cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: PDE2A phosphodiesterase 2A, cGMP-stimulated [ Homo sapiens ]
• Official Symbol: PDE2A
• Synonyms: PDE2A; phosphodiesterase 2A, cGMP-stimulated; cGMP-dependent 3,5-cyclic phosphodiesterase; cGMP-stimulated phosphodiesterase 1; cGMP-stimulated phosphodiesterase 4; cyclic GMP-stimulated phosphodiesterase; PDE2A1; PED2A4; cGSPDE; CGS-PDE
• Gene ID: 5138
• mRNA Refseq: NM_001143839
• Protein Refseq: NP_001137311
• MIM: 602658
• UniProt ID: O00408
• Chromosome Location: 11q13.1-q14.1
• Pathway: G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; Hemostasis, organism-specific biosystem; Morphine addiction, organism-specific biosystem; Morphine addiction, conserved biosystem; Nitric oxide stimulates guanylate cyclase, organism-specific biosystem; Platelet homeostasis, organism-specific biosystem
• Function: 3,5-cyclic-nucleotide phosphodiesterase activity; TPR domain binding; cAMP binding; cGMP binding; cGMP binding; cGMP-stimulated cyclic-nucleotide phosphodiesterase activity; calcium channel activity; cyclic-nucleotide phosphodiesterase activity; drug binding; drug binding; hydrolase activity; metal ion binding; nucleotide binding; phosphoric diester hydrolase activity; protein binding; protein homodimerization activity