Recombinant Human PIK3CA/PIK3R1, FLAG-tagged

Cat.No. : PIK3CA/PIK3R1-631H
Product Overview : Recombinant human p110α and p85α (no tag) complex of N-terminal FLAG-tagged were coexpressed in aBaculovirus infected Sf9 cellexpression system. p110α MW=129 kDa, p85α MW=88 kDa.
  • Specification
  • Gene Information
  • Related Products
  • Download
Cat. No. : PIK3CA/PIK3R1-631H
Description : The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110α catalytic subunit (PIK3CA) is frequently mutated or amplified in a variety of cancers including ovarian and colon and this protein is one of the PI3K mutants. PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of p110α is essential for vascular development and inactivation of p110α leads to severe defects in angiogenic sprouting and vascular remodeling.
Source : Sf9 cells.
Formulated In : 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol, and 3 mM DTT.
Specific Activity : 130 pmol/min/μg. A unit of 110a/p85a kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C. A 25 μl kinase reaction is conducted in a buffer containing 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 5 mM MgCl2, 100 μM ATP, 10 μg mix of PS and Phosphoinositol-4,5-Bisphosphate, and 50 ng enzyme for 15 minutes at 30°C. Conversion of ATP to ADP is detected using PI3K ADP-Glo® Luminescent Kinase Assay (Promega Corporation, Madison, WI).
Application : Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Stability : >6 months at –80°C.
Pathways : Calcium signaling pathway; Insulin signaling pathway; Metabolism of carbohydrates; Chronic myeloid leukemia; Pancreatic cancer; Pathways in cancer; Small cell lung cancer; T cell receptor signaling pathway; Acute myeloid leukemia; Colorectal cancer; Endometrial cancer; Fc gamma R-mediated phagocytosis; Fc epsilon RI signaling pathway; Acute myeloid leukemia; Jak-STAT signaling pathway; Leukocyte transendothelial migration; Melanoma; Natural killer cell mediated cytotoxicity; Neurotrophin signaling pathway; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Phosphatidylinositol signaling; Progesterone-mediated oocyte maturation; Prostate cancer; Regulation of actin cytoskeleton; Renal cell carcinoma; Small cell lung cancer; T cell receptor signaling pathway; Toll-like receptor signaling pathway; Type II diabetes mellitus; VEGF signaling pathway; mTOR signaling pathway
Gene Name : PIK3CA phosphoinositide-3-kinase, catalytic, alpha polypeptide [ Homo sapiens ],PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha) [ Homo sapiens ]
Synonyms : PIK3CA; phosphoinositide-3-kinase, catalytic, alpha polypeptide; PI3K; MGC142161; MGC142163; p110-alpha; Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; EC; PI3-kinase p110 subunit alpha; PtdIns-3-kinase p110; PI3Kbeta; PIK3R1; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); p85; GRB1; p85-ALPHA
Gene ID : 5290,5295
mRNA Refseq : NM_006218,NM_181504
Protein Refseq : NP_006209,NP_852556
MIM : 171834,171833
UniProt ID : P42336, P27986
Chromosome Location : 3q26.3, 5q13.1
Function : ATP binding; nucleotide binding; transferase activity; 1-phosphatidylinositol-3-kinase activity; inositol or phosphatidylinositol kinase activity; insulin receptor substrate binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphotransferase activity, alcohol group as acceptor; ErbB-3 class receptor binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.


  • Q&As
  • Reviews

Q&As (0)

Ask a question

Customer Reviews (0)

Write a review

Ask a Question for All PIK3CA Products

Required fields are marked with *

My Review for All PIK3CA Products

Required fields are marked with *


Inquiry Basket



Terms and Conditions        Privacy Policy

Copyright © 2024 Creative BioMart. All Rights Reserved.

Contact Us

  • /
  • Service lnquiry:

Stay Updated on the Latest Bioscience Trends