Recombinant Mouse and Human Pik3ca/PIK3R1, His-tagged, Active

• Cat.No.: Pik3ca/PIK3R1-390H
• Product Overview: Recombinant full-length mouse p110alpha and human p85alpha were co-expressed by baculovirus inSf9 insect cellsusing an N-terminal His tag on both proteins. MW=111 kDa (p110α), ~86 kDa (p85α).

Specification

• Species: Human/Mouse
• Source: Sf9 Cells
• Tag: His
• Description: The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110α catalytic subunit (PIK3CA) is frequently mutated or amplified in a variety of cancers including ovarian and colon and this protein is one of the PI3K mutants. PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of p110α is essential for vascular development and inactivation of p110α leads to severe defects in angiogenic sprouting and vascular remodeling .
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: Pik3ca phosphatidylinositol 3-kinase, catalytic, alpha polypeptide [ Mus musculus ],PIK3R1 phosphoinositide-3-kinase, regulatory subunit 1 (alpha) [ Homo sapiens ]
• Synonyms: Pik3ca; phosphatidylinositol 3-kinase, catalytic, alpha polypeptide; p110; caPI3K; MGC161268; p110alpha; 6330412C24Rik; PIK3R1; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); p85; GRB1; p85-ALPHA
• Gene ID: 18706,5295
• mRNA Refseq: NM_008839,NM_181504
• Protein Refseq: NP_032865,NP_852556
• UniProt ID: P42337, P27986
• Chromosome Location: 3 12.4 cM, 5q13.1
• MIM: 171833
• Pathway: Calcium signaling pathway; Insulin signaling pathway; Metabolism of carbohydrates; Chronic myeloid leukemia; Pancreatic cancer; Pathways in cancer; Small cell lung cancer; T cell receptor signaling pathway; Acute myeloid leukemia; Colorectal cancer; Endometrial cancer; Fc gamma R-mediated phagocytosis; Fc epsilon RI signaling pathway; Acute myeloid leukemia; Jak-STAT signaling pathway; Leukocyte transendothelial migration; Melanoma; Natural killer cell mediated cytotoxicity; Neurotrophin signaling pathway; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Phosphatidylinositol signaling; Progesterone-mediated oocyte maturation; Prostate cancer; Regulation of actin cytoskeleton; Renal cell carcinoma; Small cell lung cancer; T cell receptor signaling pathway; Toll-like receptor signaling pathway; Type II diabetes mellitus; VEGF signaling pathway; mTOR signaling pathway
• Function: ATP binding; nucleotide binding; transferase activity; 1-phosphatidylinositol-3-kinase activity; inositol or phosphatidylinositol kinase activity; insulin receptor substrate binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphotransferase activity, alcohol group as acceptor; ErbB-3 class receptor binding