Recombinant Human PLK4 protein, His-tagged

• Cat.No.: PLK4-2618H
• Product Overview: Recombinant Human PLK4 protein(1-263 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 1-263 aa
• AA Sequence: MATCIGEKIEDFKVGNLLGKGSFAG VYRAESIHTGLEVAIKMIDKKAMYK AGMVQRVQNEVKIHCQLKHPSILEL YNYFEDSNYVYLVLEMCHNGEMNRY LKNRVKPFSENEARHFMHQIITGML YLHSHGILHRDLTLSNLLLTRNMNI KIADFGLATQLKMPHEKHYTLCGTP NYISPEIATRSAHGLESDVWSLGCM FYTLLIGRPPFDTDTVKNTLNKVVL ADYEMPSFLSIEAKDLIHQLLRRNP ADRLSLSSVLDHP
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: PLK4 polo-like kinase 4 [ Homo sapiens ]
• Official Symbol: PLK4
• Synonyms: PLK4; polo-like kinase 4; polo like kinase 4 (Drosophila) , serine/threonine kinase 18 , STK18; serine/threonine-protein kinase PLK4; Sak; PLK-4; Snk akin kinase; serine/threonine kinase 18; serine/threonine-protein kinase 18; serine/threonine protein kinase SAK; serine/threonine-protein kinase Sak; SAK; STK18
• Gene ID: 10733
• mRNA Refseq: NM_001190799
• Protein Refseq: NP_001177728
• MIM: 605031
• UniProt ID: O00444