Recombinant Human RNASE1 Protein, His-tagged

• Cat.No.: RNASE1-394H
• Product Overview: Recombinant Human RNASE1(Lys29~Thr156) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
• Source: E. coli
• Species: Human
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 16.1kDa
• Identity: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Protein length: Lys29~Thr156
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others

Gene Information

• Gene Name: RNASE1 ribonuclease, RNase A family, 1 (pancreatic) [ Homo sapiens ]
• Official Symbol: RNASE1
• Synonyms: RNASE1; ribonuclease, RNase A family, 1 (pancreatic); RNS1; ribonuclease pancreatic; RIB-1; RNase 1; RNase A; HP-RNase; RNase upI-1; ribonuclease 1; ribonuclease A; RIB1; MGC12408
• Gene ID: 6035
• mRNA Refseq: NM_002933
• Protein Refseq: NP_002924
• MIM: 180440
• UniProt ID: P07998