Recombinant Human STAT3, GST-tagged

• Cat.No.: STAT3-1496H
• Product Overview: Recombinant full-length human STAT3 was expressed by baculovirus inSf9insect cells using an N-terminal GST tag. MW = 120 kDa.

Specification

• Cat. No.: STAT3-1496H
• Description: STAT3 is a member of the signal transducers and activators of transcription (STAT) family of proteins that carry out a dual function: signal transduction and activation of transcription. STAT3 is widely expressed and becomes activated through phosphorylation on tyrosine as a DNA binding protein in response to a variety of stimuli such as EGF, IL-6, PDGF, IL-2 and G-CSF. This phosphoprotein forms homodimers as well as heterodimers with other members of the STAT family and translocate to the nucleus in order to modulate the transcription of various genes.
• Source: Sf9 insect cells using baculovirus.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at -70℃. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Publication:
  mTORC1 Prevents Preosteoblast Differentiation through the Notch Signaling Pathway (2015)
  Blockade of Glioma Proliferation Through Allosteric Inhibition of JAK2 (2013)
  Suppression of Breast Cancer Cell Proliferation by Selective Single-Domain Antibody for Intracellular STAT3 (2018)
  A Broad-Based Characterization of a Cell-Penetrating, Single Domain Camelid Bi-Specific Antibody Monomer That Targets STAT3 and KRAS Dependent Cancers (2021)

Gene Information

• Gene Name: STAT3 signal transducer and activator of transcription 3 (acute-phase response factor) [ Homo sapiens ]
• Synonyms: STAT3; signal transducer and activator of transcription 3 (acute-phase response factor); APRF; HIES; FLJ20882; MGC16063; signal transducer and activator of transcription 3; DNA-binding protein APRF; acute-phase response factor
• Gene ID: 6774
• mRNA Refseq: NM_003150
• Protein Refseq: NP_003141
• MIM: 102582
• UniProt ID: P40763
• Chromosome Location: 17q21.31
• Pathway: Acute myeloid leukemia; Adipocytokine signaling pathway; Chemokine signaling pathway; Jak-STAT signaling pathway; Pancreatic cancer; Pathways in cancer; Signaling by PDGF; Signalling by NGF
• Function: calcium ion binding; hematopoietin/interferon-class (D200-domain) cytokine receptor signal transducer activity; protein dimerization activity; protein kinase binding; sequence-specific DNA binding; signal transducer activity; transcription activator activity; transcription factor activity; transcription factor binding

Reviews

11/17/2022

Protein worked well in assay.

04/21/2021

Fast shipping, great customer service.

02/06/2018

Excellent purity, highly recommend.

Q&As

Are there any known inhibitors targeting STAT3 for therapeutic purposes?

Yes, there are several inhibitors targeting STAT3, including Stattic, S3I-201, and OPB-31121, which are being explored for their therapeutic potential in cancer and other diseases.

Can STAT3 activation influence cell proliferation and survival?

Yes, activated STAT3 can promote cell proliferation and survival by inducing the expression of genes like cyclin D1 and Bcl-xL.

How does STAT3 contribute to oncogenesis and tumor progression?

STAT3 can contribute to oncogenesis by promoting cell proliferation, survival, angiogenesis, and immune evasion, and by suppressing apoptosis in various cancer types.

How can the activation of STAT3 be detected in cells?

Activation of STAT3 can be detected using Western blotting with phospho-specific antibodies that recognize the tyrosine-phosphorylated form of STAT3.

How does STAT3 interact with other signaling pathways in the cell?

STAT3 can interact with other signaling pathways such as the MAPK, PI3K/AKT, and NF-κB pathways, leading to integrated cellular responses.

What are the primary upstream activators of STAT3?

The primary upstream activators of STAT3 are cytokines and growth factors, especially those that signal through the Janus kinase (JAK) family, such as IL-6 and EGF.

What role does STAT3 play in immune cell differentiation and function?

STAT3 plays a crucial role in the differentiation of Th17 cells, a subset of CD4+ T cells, and is also involved in the function of myeloid-derived suppressor cells (MDSCs) and regulatory T cells, influencing immune responses.