Recombinant Human TSPO, His-tagged

• Cat.No.: TSPO-3462H
• Product Overview: Recombinant Human TSPO protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-169aa
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.
• Publications:
  Concentration, distribution, and influence of aging on the 18 kDa translocator protein in human brain: Implications for brain imaging studies (2019)

Gene Information

• Gene Name: TSPO translocator protein (18kDa) [ Homo sapiens ]
• Official Symbol: TSPO
• Synonyms: TSPO; translocator protein (18kDa); benzodiazapine receptor (peripheral) , BZRP; translocator protein; DBI; IBP; MBR; mDRC; PBR; peripheral type benzodiazepine receptor/recognition site; pk18; PKBS; mitochondrial benzodiazepine receptor; benzodiazepine peripheral binding site; peripheral-type benzodiazepine receptor; PBS; BPBS; BZRP; PTBR
• Gene ID: 706
• mRNA Refseq: NM_000714
• Protein Refseq: NP_000705
• MIM: 109610
• UniProt ID: B1AH88
• Chromosome Location: 22q13.3
• Pathway: HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem
• Function: androgen binding; benzodiazepine receptor activity; cholesterol binding; receptor activity

Citation

Concentration, distribution, and influence of aging on the 18 kDa translocator protein in human brain: Implications for brain imaging studies

Journal: Journal of Cerebral Blood Flow & Metabolism    PubMed ID: 31220997    Data: 2020/5/1

Authors: Junchao Tong, Belinda Williams, Stephen J Kish

Article Snippet:No significant correlation (Pearson) was observed between levels of either TSPO or GFAP protein and PMI of the subjects examined. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Supplier Cat# Isotype Epitope Working dilution Comments Antibodies TSPO1 (18 kDa, 169aa) Abcam ab109497 rMonoAb (EPR5384) aa150-169 1:100K Good reactivity & selectivity for 18 kDa TSPO ThermoFisher MA5-24844 rMonoAb (4H2) C-terminal 1:30K Good reactivity & selectivity for 18 kDa TSPO ThermoFisher PA5-75544 rPolyAb 124-169 1:10K Some cross reactivity at higher MW Bio-Rad VPA-00789 rPolyAb C-terminal 1:2K Low reactivity & selectivity for human brain TSPO Origene TA303138 gPolyAb aa156-169 NA Major band at 35 kDa?. Abnova H00000706-M01 Mouse IgG 1k (3D8-B2) Unknown NA No major band identified Santa Cruz Biotech Sc-20120 rPolyAb (FL-169) Unknown NA No major band identified GFAP (50,48,45,and 43 kDa) Chemicon/Millipore MAB360 Mouse IgG 1 Unknown 1:100K Vimentin (57 kDa) Santa Cruz Biotech Sc-6260 Mouse IgG 1 Unknown 1:2K 2 nd antibodies Anti-rabbit IgG-HRP Southern Biotech 4050-05 Goat IgG 1:10-20K Anti-mouse IgG1-HRP Southern Biotech 1070-05 Goat IgG 1:10K Anti-goat IgG-HRP Southern Biotech 6160-05 Rabbit IgG 1:10K Recombinant TSPO Human TSPO, N-His Creative BioMart TSPO-3462H / / 0.1 ng 21 kDa Human TSPO, N-His/GST Creative BioMart TSPO-1696H / / <1 ng 48.8 kDa (crude lysate) Human TSPO, C-Myc/DDK Origene TP320107 / / 40 ng 23 kDa Mouse TSPO, N-His Jean J. Lacapere Gift / / 1 ng 21 kDa Mouse TSPO, C-Myc/DDK Origene TP605624 / / NA 23 kDa Rat TSPO, C-Myc/DDK Origene TP605625 / / NA 23 kDa Open in a separate window Antibody and recombinant protein sources and conditions used.. Data analyses All quantitative immunoblotting data are expressed in ng/μg protein of tissue homogenates.

Antibody and recombinant protein sources and conditions used.

Quantification of TSPO protein in autopsied human brain. (a) Immunoblots of TSPO in the pooled tissue standards, in the commercial N-His-tagged recombinant human TSPO-3462H and in human adrenal samples with monoclonal (EPR5384, 1:100K and MA5-24844, 1:30K dilution) and polyclonal (PA5-75544, 1:10K) antibodies. (b) Standard curves for the recombinant TSPO. Note more abundant TSPO in adrenal than in brain for both EPR5384 and MA5-24844, polymer protein bands of the recombinant TSPO, high molecular weight non-specific reactions for PA5-75544, and detection of a TSPO fragment but not 18 kDa TSPO by PA5-75544 in human adrenal samples.