Recombinant Human TUBA1A Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human TUBA1A Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : TUBA1A-5777H
Product Overview : TUBA1A MS Standard C13 and N15-labeled recombinant protein (NP_006000) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blot studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, intellectual disability, and early-onset epilepsy caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 50.1 kDa
AA Sequence : MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK HVPRAVFVDL EPTVIDEVRT GTYRQLFHPE QLITGKEDAA NNYARGHYTI GKEIIDLVLD RIRKLADQCT GLQGFLVFHS FGGGTGSGFT SLLMERLSVD YGKKSKLEFS IYPAPQVSTA VVEPYNSILT THTTLEHSDC AFMVDNEAIY DICRRNLDIE RPTYTNLNRL IGQIVSSITA SLRFDGALNV DLTEFQTNLV PYPRIHFPLA TYAPVISAEK AYHEQLSVAE ITNACFEPAN QMVKCDPRHG KYMACCLLYR GDVVPKDVNA AIATIKTKRT IQFVDWCPTG FKVGINYQPP TVVPGGDLAK VQRAVCMLSN TTAIAEAWAR LDHKFDLMYA KRAFVHWYVG EGMEEGEFSE AREDMAALEK DYEEVGVDSV EGEGEEEGEE YTRTRPLEQK LISEEDLAAN DILDYKDDDD KV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : TUBA1A tubulin alpha 1a [ Homo sapiens (human) ]
Official Symbol : TUBA1A
Synonyms : TUBA1A; tubulin, alpha 1a; tubulin alpha-1A chain; B ALPHA 1; FLJ25113; TUBA3; tubulin; alpha; brain specific; hum-a-tub1; hum-a-tub2; alpha-tubulin 3; tubulin, alpha 3; tubulin B-alpha-1; tubulin alpha-3 chain; tubulin, alpha, brain-specific; LIS3; B-ALPHA-1;
Gene ID : 7846
mRNA Refseq : NM_006009
Protein Refseq : NP_006000
MIM : 602529
UniProt ID : Q71U36

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