Recombinant Mouse Cdk1, His-GST tagged
Cat.No. : | CDK1-402M |
Product Overview : | Recombinant Mouse Cdk1 (P11440) (Met1-Met297), fused with the N-terminal polyhistidine-tagged GST tag, was produced in Baculovirus-Insect cells. |
Availability | February 10, 2025 |
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Source : | Baculovirus-Insect cells |
Species : | Mouse |
Tag : | His-GST |
Predicted N Terminal : | Met |
Form : | Lyophilized from sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH 8.0.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. |
Molecular Mass : | The recombinant mouse CDK1/GST chimera consists of 534 amino acids and has a calculated molecular mass of 61.9 kDa. The recombinant protein migrates as an approximately 57 kDa band in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 eu per μg of the protein as determined by the LAL method. |
Purity : | >85 % as determined by SDS-PAGE |
Stability : | Samples are stable for up to twelve months from date of receipt at -70oC. |
Storage : | Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Protein length : | Met1-Met297 |
Gene Name : | Cdk1 cyclin-dependent kinase 1 [ Mus musculus ] |
Official Symbol : | Cdk1 |
Gene ID : | 12534 |
mRNA Refseq : | NM_007659 |
Protein Refseq : | NP_031685 |
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Related Gene
Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis
Journal: The Journal of Cell Biology PubMed ID: 29074707 Data: 2018/1/2
Authors: Bernhard Strauss, Andrew Harrison, Jonathon Pines
Article Snippet:Recombinant mouse His-GST CDK1 and recombinant mouse 10xHis CCNB1Myc (Creative BioMart) were used as protein standards and loaded at 1, 5, 10 ng per lane.. Wild-type mouse embryo samples containing 130 uninjected embryos and 130 embryos injected with CyclinB1–Venus mRNA at the zygote stage were harvested at the two-cell stage, and embryo extracts were prepared using lysis buffer (150 mM KCl, 20 mM Hepes, pH 7.6, 2 mM EGTA, 1.5 mM MgCl 2 , 50 mM NaF, 0.1% NP-40, 10% glycerol, 1 mM Na3VO4, 20 mM β-glycerophosphate, 1 mM dithiothreitol, 10 mM benzamidine HCl, and 25 U/ml Benzonase nuclease [Merck]) supplemented with Protease inhibitor cocktail (P2714; Sigma-Aldrich).Wild-type mouse embryo samples containing 130 uninjected embryos and 130 embryos injected with CyclinB1–Venus mRNA at the zygote stage were harvested at the two-cell stage, and embryo extracts were prepared using lysis buffer (150 mM KCl, 20 mM Hepes, pH 7.6, 2 mM EGTA, 1.5 mM MgCl 2 , 50 mM NaF, 0.1% NP-40, 10% glycerol,..

Low amounts of Cyclin B1 are sufficient to trigger mitosis and the threshold is reached by mid S phase. (A) To establish the threshold of Cyclin B1–Venus that can drive null cells into mitosis, fluorescence intensity was used as a read-out for the amount of protein and measured in individual blastomeres of ?/? embryos; y axis arbitrary intensity units. The threshold concentration to trigger mitosis lies between 100 and 200 units above background intensity, corresponding to ~1/10 of the sampled intensity range ( n = 21 blastomeres from five experiments). (B) Wild-type Cyclin B1–Venus was injected into one blastomere of ?/? embryos at the two-cell stage to rescue the arrest phenotype. Signal intensity was measured at the 8- to 16-cell transition after addition of Wee1 inhibitor. Only injected cells with a Cyclin B1 level above a threshold of ~150 arbitrary units can be forced into mitosis by Wee1 inhibition; n = 17, 1 experiment. Error bars are the SD of the mean. (C–F) To test whether there is a time dependency of the inhibitory phosphorylation of

Tethering Cyclin
Not For Human Consumption!
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Customer Reviews (3)
Write a reviewThe product brochure is very detailed and has comprehensive basic information about the CDK1 protein.
The staff actively communicated with me and solved many technical problems.
Very professional customer service, fully understanding the needs of the customer and giving professional advice.
Q&As (4)
Ask a questionCDK1 is involved in papillary thyroid cancer, hepatocellular carcinoma, and other diseases.
CDK1 is positively correlated with IL⁃33 expression and CDK1 and IL⁃33 expression interact with each other.
CDK1 is an important regulator of the eukaryotic cell cycle that drives cell cycle progression by binding to cell cycle proteins.
CDK1 expression levels were higher in CRC tissues than in paracancerous tissues.
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