Active Recombinant Full Length Human CDK1 Protein, GST-tagged

• Cat.No.: CDK1-2418HF
• Product Overview: Full-length human CDK1 [1-297(end) amino acids of accession number NP_001777.1] was co-expressed as N-terminal GST-fusion protein (61 kDa) with CyclinB1 [1-433(end) amino acids of accession number NP_114172.1] using baculovirus expression system. GST-CDK1 was purified by using glutathione sepharose chromatography.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: GST
• Protein Length: 1-297
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.
• Purity: 80%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: cyclin-dependent kinase 1
• Official Symbol: CDK1
• Synonyms: CDK1; CDC2; CDC28A; P34CDC2
• Protein Refseq: NP_001777.1
• UniProt ID: P06493