Active Recombinant Full Length Human CDK1 Protein, DDK-tagged, Biotinylated

• Cat.No.: CDK1-3397HF
• Product Overview: Full-length human CDK1 [1-297(end) amino acids of accession number NP_001777.1] was co-expressed as N-terminal DYKDDDDK tagged, biotinylated protein (38 kDa) with His-CyclinA2 [1-432(end) amino acids of accession number NP_001228.2] using baculovirus expression system. The protein was purified by using DYKDDDDK tag antibody agarose.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: DDK
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.
• Protein length: 1-297(end)
• Purity: 90%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: cyclin-dependent kinase 1
• Official Symbol: CDK1
• Synonyms: CDK1; CDC2; CDC28A; P34CDC2
• Protein Refseq: NP_001777.1
• UniProt ID: P06493