Recombinant Mouse Cpe, His-tagged

• Cat.No.: Cpe-214M
• Product Overview: Recombinant Mouse CPE MS Standard Protein, C13 and N15-labeled (Mouse CPE, Heavy Labeled) Gln 28 - Phe 476 (Accession # NP_038522) was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain >99% incorporation effi

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 28-476 a.a.
• Description: Carboxypeptidase E (CPE) is also known as Carboxypeptidase H or enkephalin convertase. Carboxypeptidase E cleaves C-terminal amino acid residues and is involved in neuropeptide processing. It is a peripheral membrane protein. CPE specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Carboxypeptidase E appears to have several functions. The active form of carboxypeptidase E was shown to be in secretory vesicles, where it acts as an exopeptidase to activate neuropeptides, It does that by cleaving off basic C-terminal amino acids, producing the active form of the peptide. Products of carboxypeptidase E include insulin, enkephalin, and most other neuroendocrine peptides.It has also been proposed that membrane-associated carboxypeptidase E acts as a sorting signal for regulated secretory proteins in the trans-Golgi network of the pituitary and in secretory granules; regulated secretory proteins are mostly hormones and neuropeptides. Mice with mutant carboxypeptidase E Cpe, display endocrine disorders like obesity and infertility.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Molecular Mass: Mouse CPE, Heavy Labeled is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 52 kDa. The predicted N-terminus is Gln 28. DTT-reduced Protein migrates as 52 kDa in SDS-PAGE .Mouse CPE, Heavy Labeled is labeled with [U- 13C6, 15N4
• Endotoxin: Less than 1.0 EU per μg of the Mouse CPE, Heavy Labeled by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
• Reconstitution: See Certificate of Analysis for reconstitution instructions and specific concentrations.

Gene Information

• Gene Name: Cpe carboxypeptidase E [ Mus musculus ]
• Official Symbol: Cpe
• Synonyms: CPE; carboxypeptidase E; carboxypeptidase H; enkephalin convertase; prohormone-processing carboxypeptidase; CPH; fat; Cph1; Cph-1; R74677; MGC7101
• Gene ID: 12876
• mRNA Refseq: NM_013494
• Protein Refseq: NP_038522
• Pathway: Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Insulin Synthesis and Processing, organism-specific biosystem; Type I diabetes mellitus, organism-specific biosystem; Type I diabetes mellitus, conserved biosystem
• Function: carboxypeptidase activity; cell adhesion molecule binding; cobalt ion binding; hydrolase activity; metal ion binding; metallocarboxypeptidase activity; metallopeptidase activity; neurexin family protein binding; peptidase activity; zinc ion binding