Active Recombinant Human CPE, His-tagged

• Cat.No.: CPE-723H
• Product Overview: Recombinant Human CPE (Accession # NP_001864) Arg42-Ser453, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Arg42
• Form: Supplied as a 0.2 µm filtered solution in MES and NaCl.
• Bio-activity: Measured by its ability to cleave a peptide substrate, benzoyl-AR-OH. The product, Arg, is reacted with ortho-phthaldialdehyde (OPA) to form a fluorescent molecule. The specific activity is >12,000 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human CPE, His-tagged has a calculated MW of 47 kDa. In SDS-PAGE migrates as 57 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Protein length: Arg42-Ser453

Gene Information

• Gene Name: CPE carboxypeptidase E [ Homo sapiens ]
• Official Symbol: CPE
• Synonyms: CPE; carboxypeptidase E; carboxypeptidase H; cobalt stimulated chromaffin granule carboxypeptidase; enkephalin convertase; insulin granule associated carboxypeptidase; CPH; prohormone-processing carboxypeptidase; insulin granule-associated carboxypeptidase; cobalt-stimulated chromaffin granule carboxypeptidase
• Gene ID: 1363
• mRNA Refseq: NM_001873
• Protein Refseq: NP_001864
• MIM: 114855
• UniProt ID: P16870
• Chromosome Location: 4q32.3