Recombinant Mouse Efnb2 Protein, His-tagged

• Cat.No.: Efnb2-367M
• Product Overview: Recombinant mouse EFNB2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Description: EFNB2, also known as ephrin-B2, is cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. It binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. It may play a role in constraining the orientation of longitudinally projecting axons.
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 23.4kDa (212aa)28-40KDa (SDS-PAGE under reducing conditions.)
• AA Sequence: RSIVLEPIYW NSSNSKFLPG QGLVLYPQIG DKLDIICPKV DSKTVGQYEY YKVYMVDKDQ ADRCTIKKEN TPLLNCARPD QDVKFTIKFQEFSPNLWGLE FQKNKDYYII STSNGSLEGL DNQEGGVCQT RAMKILMKVG QDASSAGSAR NHGPTRRPEL EAGTNGRSST TSPFVKPNPGSSTDGNSAGH SGNNLLGSEV ALFALEHHHH HH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: Efnb2 ephrin B2 [ Mus musculus ]
• Official Symbol: Efnb2
• Synonyms: EFNB2; ephrin B2; ephrin-B2; HTK ligand; EPH-related receptor tyrosine kinase ligand 5; Epl5; ELF-2; Eplg5; Htk-L; Lerk5; LERK-5; NLERK-1
• Gene ID: 13642
• mRNA Refseq: NM_010111
• Protein Refseq: NP_034241
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem
• Function: ephrin receptor binding; protein binding; receptor binding