Recombinant Mouse Eif2ak3 protein, His & T7-tagged

• Cat.No.: Eif2ak3-1392M
• Product Overview: Recombinant Mouse Eif2ak3 aa. (Met973~Asn1114 (Accession # Q9Z2B5) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His&T7
• Protein Length: Met973~Asn1114
• Description: The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in a similar gene in human are associated with Wolcott-Rallison syndrome.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 19.9kDa.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 7.9.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Eif2ak3 eukaryotic translation initiation factor 2 alpha kinase 3 [ Mus musculus (house mouse) ]
• Official Symbol: Eif2ak3
• Synonyms: Eif2ak3; eukaryotic translation initiation factor 2 alpha kinase 3; Pek; Perk; eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; pancreatic eIF2-alpha kinase
• Gene ID: 13666
• mRNA Refseq: NM_001313918.1
• Protein Refseq: NP_001300847.1
• UniProt ID: Q9Z2B5