Recombinant Mouse Fgf21

• Cat.No.: Fgf21-504M
• Product Overview: Total 183 AA. MW: 20.1 kDa (calculated). 182 AA of the mouse FGF21 and one extra AA, N-terminal methionin (highlighted).

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Description: Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.
• Form: Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 20mM NaCl, pH 7.5.
• Purity: Purity>95%
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. Add DTT (0.2 mM ) and NaCl (0.1-0.15 M) before freezing to prevent potential aggregation.
• Applications: ELISA, Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
• Sequences of amino acids: MAYPIPDSSP LLQFGGQVRQ RYLYTDDDQD TEAHLEIRED GTVVGAAHRS PESLLELKAL KPGVIQILGV KASRFLCQQP DGALYGSPHF DPEACSFREL LLEDGYNVYQ SEAHGLPLRL PQKDSPNQDA TSWGPVRFLP MPGLLHEPQD QAGFLPPEPP DVGSSDPLSM VEPLQGRSPS YAS
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Gene Information

• Gene Name: Fgf21 fibroblast growth factor 21 [ Mus musculus ]
• Official Symbol: Fgf21
• Synonyms: Fgf21; fibroblast growth factor 21
• Gene ID: 56636
• mRNA Refseq: NM_020013
• Protein Refseq: NP_064397
• UniProt ID: Q9JJN1
• Chromosome Location: 7B2
• Pathway: ESC Pluripotency Pathways; MAPK signaling pathway; Melanoma; Pathways in cancer; Regulation of Actin Cytoskeleton; Regulation of actin cytoskeleton
• Function: growth factor activity

Citation

Alcoholic fatty liver is enhanced in CYP2A5 knockout mice: the role of the PPARα-FGF21 axis

Journal: Toxicology    PubMed ID: 28131861    Data: 2017/1/25

Authors: Xue Chen, Stephen C. Ward, Yongke Lu

Article Snippet:The amount of food consumed by the different genetically mutated mice used in this study was approximately the same.The amount of food consumed by the different genetically mutated mice used in this study was approximately the same.. Some mice were daily injected mouse recombinant FGF21 (rFGF21, purchased from Creative BioMart, Shirley, NY, USA) subcutaneously at 0.25 mg/kg body weight.. The mice were fasted for 6 h and then sacrificed by cervical dislocation after blood was collected via the retro-orbital venous sinus under anesthesia by inhalation of isoflurane.The mice were fasted for 6 h and then sacrificed by cervical dislocation after blood was collected via the retro-orbital venous sinus under anesthesia by inhalation of isoflurane.

Chronic ethanol induces FGF21 in Cyp2a5+/+ mice but does not in Cyp2a5?/? mice. (A) Serum FGF21 (N=5). (B) Liver expression of FGF21 and PPARα by Western blotting analysis. (C) Quantitation of Western blots (N=4). *P<0.05, compared to Cyp2a5+/+ Control Group. Cont, Control; EtOH, Ethanol.

Chronic ethanol induces FGF21 in Nrf2+/+ mice more than in Nrf2?/? mice. (A) Western blots. (B) Quantitation of Western blots (N=3). *P<0.05, compared to Nrf2+/+ Control Group; # P<0.05, compared to Nrf2?/? Control Group; & P<0.05, compared with Nrf2+/+ Ethanol group. Cont, Control; EtOH, Ethanol.

Recombinant FGF21 blunts ethanol-induced hypertriglyceridemia (HTG) in Pparα?/? mice. (A) Ethanol induction of FGF21 was blunted in Pparα?/? mice (N=5). (B) rFGF21 blunted ethanol-induced elevation of liver TG in WT mice but not in Pparα?/? mice (N=5). (C) rFGF21 blunted ethanol-induced HTG in Pparα?/? mice (N=5). (D) Ethanol induction of CYP2E1 and CYP2A5 was not affected in both Pparα+/+ and Pparα?/? mice (N=5). *P<0.05, compared to Control Group. # P<0.05, compared to WT Ethanol Group. $ P<0.05, compared to WT Control Group. ^ P<0.05, compared with PPARα KO Ethanol group. E+F, ethanol plus rFGF21; WT, wild-type; PPARα KO, Pparα?/?.