Active Recombinant Mouse Fgf21 protein, His-tagged, Low Endotoxin

• Cat.No.: Fgf21-574M
• Product Overview: Recombinant Mouse Fgf21 protein(Q9JJN1)(29-210 aa), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 29-210 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Mouse FGF21, His Tag immobilized on CM5 Chip can bind Human Beta Klotho, His Tag with an affinity constant of 41.50 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 21 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Fgf21 fibroblast growth factor 21 [ Mus musculus ]
• Official Symbol: Fgf21
• Synonyms: FGF21; fibroblast growth factor 21; FGF-21
• Gene ID: 56636
• mRNA Refseq: NM_020013
• Protein Refseq: NP_064397