Description : |
This gene encodes an anti-inflammatory cytokine that is a member of the class-2 cytokine family. The encoded protein is secreted by cells of both the innate and adaptive immune systems and is crucial for limiting the immune response to a broad range of pathogens. It also has been shown to suppress autoimmune responses. This protein mediates it's immunosuppressive signal through a specific interleukin 10 receptor complex. Aberrant functioning of this gene is associated with numerous immune disorders including graft-versus-host disease, and increased susceptibility to HIV-1 infection and rheumatoid arthritis. |
Source : |
E. coli |
Species : |
Mouse |
Bio-activity : |
The ED50 is 1- 2 ng/ml, corresponding to a specific activity of 1- 0.5 x 10^6 units/mg, determined by the dose dependent stimulation MC/9 cell proliferation. |
Molecular Mass : |
18.9 kDa |
Endotoxin : |
Less than 0.01 ng per µg protein as determined by the LAL method |
Purity : |
>98%, as determined by Coomassie stained SDS-PAGE. |
Stability : |
Unopened vial can be stored between 2 centigrade and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 centigrade and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
Storage : |
Store at -80 centigrade. |
Concentration : |
Protein formulated and vialed at >50 ug/mL in specified buffer |
Storage Buffer : |
20mM Tris pH7.5 |