| Cat.No. : |
Il10-083M |
| Product Overview : |
Purified recombinant protein of Mouse interleukin 10 (Il10) (Ser19-Ser178) without tag was expressed in E.coli. |
| Description : |
This gene encodes an anti-inflammatory cytokine that is a member of the class-2 cytokine family. The encoded protein is secreted by cells of both the innate and adaptive immune systems and is crucial for limiting the immune response to a broad range of pathogens. It also has been shown to suppress autoimmune responses. This protein mediates it's immunosuppressive signal through a specific interleukin 10 receptor complex. Aberrant functioning of this gene is associated with numerous immune disorders including graft-versus-host disease, and increased susceptibility to HIV-1 infection and rheumatoid arthritis. |
| Source : |
E. coli |
| Species : |
Mouse |
| Bio-activity : |
The ED50 is 1- 2 ng/ml, corresponding to a specific activity of 1- 0.5 x 10^6 units/mg, determined by the dose dependent stimulation MC/9 cell proliferation. |
| Molecular Mass : |
18.9 kDa |
| Endotoxin : |
Less than 0.01 ng per µg protein as determined by the LAL method |
| Purity : |
>98%, as determined by Coomassie stained SDS-PAGE. |
| Stability : |
Unopened vial can be stored between 2 centigrade and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 centigrade and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
| Storage : |
Store at -80 centigrade. |
| Concentration : |
Protein formulated and vialed at >50 ug/mL in specified buffer |
| Storage Buffer : |
20mM Tris pH7.5 |
| Figure : |
|
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.