Active Recombinant Mouse IL4 Protein

• Cat.No.: IL4-179M
• Product Overview: Recombinant Mouse IL4 Protein without tag was expressed in E. coli.

Specification

• Description: Interleukin 4 (IL-4) is an immunomodulatory cytokine that functions to induce naïve helper T cells to differentiate into type 2 T helper (Th2) cells. Th2 cells subsequently produce more IL-4 in a positive feedback loop. IL-4 also promotes immunoglobulin IgG to IgE isotype switching on B cells. IL-4 binds the IL-4Rα receptor to activate STAT6 signaling.
• Source: E. coli
• Species: Mouse
• Bio-activity: HT-2 proliferation, ≤20 ng/mL; ≥5.0 x 10^4 units/mg
• Molecular Mass: Monomer, 13.7 kDa (121 aa)
• AA Sequence: MHIHGCDKNHLREIIGILNEVTGEG TPCTEMDVPNVLTATKNTTESELVC RASKVLRIFYLKHGKTPCLKKNSSV LMELQRLFRAFRCLDSSISCTMNES KSTSLKDFLESLKSIMQMDYS
• Endotoxin: ≤1 EUs/μg, Kinetic LAL
• Purity: ≥95%, Reducing and Non-Reducing SDS PAGE
• Stability: 12 months from date of receipt when stored at -20 to -80 centigrade as supplied.
  1 month when stored at 4 centigrade after reconstituting as directed.
  3 months when stored at -20 to -80 centigrade after reconstituting as directed.
• Storage: Storage Prior to Reconstitution: -20 centigrade
• Storage Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
• Reconstitution: Sterile water at 0.1 mg/mL
• Shipping: Room temperature
• Instructions: Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80 centigrade and avoid repeat freeze thaws.

Gene Information

• Gene Name: Il4 interleukin 4 [ Mus musculus (house mouse) ]
• Official Symbol: IL4
• Synonyms: IL4; interleukin 4; interleukin-4; IGG1 induction factor; B-cell growth factor 1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell IgG differentiation factor; Il-4; BSF-1
• Gene ID: 16189
• mRNA Refseq: NM_021283
• Protein Refseq: NP_067258
• UniProt ID: P07750

Reviews

10/17/2021

By testing immunogenicity in animal models, detect whether an immune response is induced.

11/09/2020

The structure of the recombinant protein was analyzed by nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy, and the results showed that the protein was stable.

05/06/2020

This protein has good stability, and the test results of heat resistance, acid and alkali resistance and protease degradation resistance are good.