Recombinant Human Interleukin 4

• Cat.No.: IL4-59H
• Product Overview: Recombinant Human Interleukin-4 produced inCHOis a single, non-glycosylated polypeptide chain containing 129 amino acids and having a molecular mass of 14 kDa, the glycosilation of IL-4 migrates as 18 kDa on SDS-PAGE. The IL-4 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: IL4-59H
• Description: IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
• Source: Chinese Hamster Ovarian Cells.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Gly-Gln-Thr.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: Lyophilized from a Phosphate- Buffered Saline (pH 7.4).
• Solubility: It is recommended to reconstitute the lyophilized Interleukin-4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is 0.05-0.2 ng/ml.
• Storage: Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IL4 should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: IL4 interleukin 4 [ Homo sapiens ]
• Synonyms: IL4; interleukin 4; BSF1; IL-4; BCGF1; BCGF-1; MGC79402; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; Lymphocyte stimulatory factor 1; B-cell stimulatory factor 1; Pitrakinra; Binetrakin; BSF-1
• Gene ID: 3565
• mRNA Refseq: NM_000589
• Protein Refseq: NP_000580
• MIM: 147780
• UniProt ID: P05112
• Chromosome Location: 5q31.1
• Pathway: T cell receptor signaling pathway; Jak-STAT signaling pathway; Fc epsilon RI signaling pathway; Cytokine-cytokine receptor interaction; Autoimmune thyroid disease; Allograft rejection; Asthma; Hematopoietic cell lineage
• Function: cytokine activity; growth factor activity; protein binding; interleukin-4 receptor binding