Recombinant Human Interleukin 4, His-tagged

• Cat.No.: IL4-137H
• Product Overview: Recombinant Interleukin-4 protein, fused to His-tag at N-terminus, was expressed inE.coliand was purified by conventional chromatography techniques, after refolding of theisolated inclusion bodies in a renaturation buffer. MW = 17.2kDa (150aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Interleukin-4 (IL-4) is a pleiotropic cytokine that has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of naïve helper T-cell to Th2 cells. Interleukin-4 is a co-stimulator of DNA-synthesis and is induces the expression of class II MHC molecules on resting B-cells. It also enhances both secretion and cell surface expression of IgE and IgG1.
• Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: IL4 interleukin 4 [ Homo sapiens ]
• Synonyms: IL4; interleukin 4; BSF1; IL-4; BCGF1; BCGF-1; MGC79402; B-cell stimulatory factor 1; Interleukin-4; Lymphocyte stimulatory factor 1; Binetrakin; Pitrakinra; B-cell stimulatory factor 1; B_cell stimulatory factor 1
• Gene ID: 3565
• mRNA Refseq: NM_000589
• Protein Refseq: NP_000580
• MIM: 147780
• UniProt ID: P05112
• Chromosome Location: 5q31.1
• Pathway: Allograft rejection; Asthma; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Hematopoietic cell lineage; Jak-STAT signaling pathway; cell receptor signaling pathway
• Function: cytokine activity; growth factor activity; interleukin-4 receptor binding; protein binding