Recombinant Pig Interleukin 1, Alpha
|Product Overview :||Recombinant Pig IL1Ahas a MW of 17.4 kDa.|
- Gene Information
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|Cat. No. :||IL1A-5432P|
|Description :||IL1A is a protein that in humans is encoded bythe IL1A gene. The protein encoded by this gene is a cytokine of theinterleukin-1 family. IL1A possesses a wide spectrum of metabolic,physiological, haematopoietic activities, and plays one of the central rolesin the regulation of the immune responses. It binds to the interleukin-1receptor. IL1A is produced mainly by activatedmacrophages, as well as neutrophils, epithelial cells, and endothelial cells.In general, Interleukin 1 is responsible for the production of inflammation,as well as the promotion of fever and sepsis.|
|Source :||E. coli|
|Form :||Filtered through a0.22 micron sterile filter. Then lyophilized, carrier-free.|
|Molecular Weight :||17.4 kDa|
|Purity :||>95% asdetermined by SDS-PAGE. Purified via sequential chromatography.|
|Endotoxin Level :||<0.1 ng/µg ofprotein|
|Biological Activity :||Determined by theD10S cell line proliferation assay with MTS detection. The ED50 is 0.02-0.04ng/ml.|
|Reconstitution :||Centrifuge vialprior to opening. Lyophilized IL-1alpha may be reconstituted in water, orother appropriate buffered solutions to a concentration of 0.1-1.0 mg/ml.These stock solutions should be apportioned into working aliquots and storedat -20°C to -80°C (not a frost-free refrigerator). Further dilution should bemade in medium or buffered solution containing tissue culture grade carrierprotein, such as PBS with 0.1-1.0% BSA. Please note that the addition of anycarrier protein into this product may produce unwanted endotoxin. Thisdepends upon the particular application employed.|
|Storage :||Lyophilized IL-1alpha should be stored at 2-4°C, preferably desiccated. Store reconstitutedIL-1 alpha at -20°C to -80°C (not in a frost-free refrigerator). When stored properly,IL-1 alpha is stable for greater than one year lyophilized and greater than 3months reconstituted. Avoid repeated freeze-thaw cycles.|
|Gene Name :||IL1A interleukin 1, alpha [ Susscrofa ]|
|Synonyms :||IL1A; interleukin 1,alpha; IL-1alpha; interleukin-1 alpha; IL-1 alpha; interleukin 1-alpha|
|Gene ID :||397094|
|mRNA Refseq :||NM_214029|
|Protein Refseq :||NP_999194|
|UniProt ID :||P18430|
|Chromosome Location :||3q12-q13; 3|
|Pathway :||Apoptosis;Cytokine-cytokine receptor interaction; Graft-versus-host disease;Hematopoietic cell lineage; Influenza A; Leishmaniasis; MAPK signalingpathway; Measles; Osteoclast differentiation; Pertussis; Prion diseases;Rheumatoid arthritis; Salmonella infection; Tuberculosis; Type I diabetesmellitus|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
Cross-talk between IL-1α's and other cytokines is dissected through methods such as co-immunoprecipitation, ELISA, and cytokine profiling arrays.
IL-1α's paradoxical roles are dissected using conditional knockout mice, 3D culture systems, and molecular analyses to delineate context-specific effects.
Single-cell RNA sequencing uncovers heterogeneity in IL-1α-responsive cell populations and their roles, revealing distinct molecular profiles and functions.
IL-1α's intricate pro-inflammatory signaling pathways are decoded using advanced techniques like mass spectrometry, phosphoproteomics, and bioinformatics.
Genetic editing tools like CRISPR-Cas9 unveil IL-1α's contributions to diseases by generating knockout models and studying resulting phenotypic changes.
In vitro co-culture systems are manipulated to study IL-1α-mediated responses, elucidating immune-stromal cell interactions via cytokine-specific neutralization.
Specific cellular receptors and effectors influenced by IL-1α are identified through techniques like co-immunoprecipitation, ChIP-seq, and siRNA knockdown.
Live-cell microscopy captures real-time IL-1α release dynamics and its impact on neighboring cells, facilitated by fluorescent tagging and high-resolution imaging.
Longitudinal studies and statistical modeling establish the quantitative relationship between IL-1α levels and disease progression, offering predictive insights.
Multi-omics analyses provide a holistic view of IL-1α signaling's impact, integrating transcriptomics, proteomics, and metabolomics data for comprehensive insights.
Customer Reviews (3)Write a review
High solubility. -
Good for intracellular staining. -
Effective in apoptosis assays. -
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