| Cat. No. : |
IL1A-5432P |
| Description : |
IL1A is a protein that in humans is encoded bythe IL1A gene. The protein encoded by this gene is a cytokine of theinterleukin-1 family. IL1A possesses a wide spectrum of metabolic,physiological, haematopoietic activities, and plays one of the central rolesin the regulation of the immune responses. It binds to the interleukin-1receptor. IL1A is produced mainly by activatedmacrophages, as well as neutrophils, epithelial cells, and endothelial cells.In general, Interleukin 1 is responsible for the production of inflammation,as well as the promotion of fever and sepsis. |
| Source : |
E. coli |
| Species : |
Pig |
| Form : |
Filtered through a0.22 micron sterile filter. Then lyophilized, carrier-free. |
| Molecular Weight : |
17.4 kDa |
| Purity : |
>95% asdetermined by SDS-PAGE. Purified via sequential chromatography. |
| Endotoxin Level : |
<0.1 ng/µg ofprotein |
| Biological Activity : |
Determined by theD10S cell line proliferation assay with MTS detection. The ED50 is 0.02-0.04ng/ml. |
| Reconstitution : |
Centrifuge vialprior to opening. Lyophilized IL-1alpha may be reconstituted in water, orother appropriate buffered solutions to a concentration of 0.1-1.0 mg/ml.These stock solutions should be apportioned into working aliquots and storedat -20°C to -80°C (not a frost-free refrigerator). Further dilution should bemade in medium or buffered solution containing tissue culture grade carrierprotein, such as PBS with 0.1-1.0% BSA. Please note that the addition of anycarrier protein into this product may produce unwanted endotoxin. Thisdepends upon the particular application employed. |
| Storage : |
Lyophilized IL-1alpha should be stored at 2-4°C, preferably desiccated. Store reconstitutedIL-1 alpha at -20°C to -80°C (not in a frost-free refrigerator). When stored properly,IL-1 alpha is stable for greater than one year lyophilized and greater than 3months reconstituted. Avoid repeated freeze-thaw cycles. |
| OfficialSymbol : |
IL1A |
