Recombinant Pig Interleukin 1, Alpha

Cat.No. : IL1A-5432P
Product Overview : Recombinant Pig IL1Ahas a MW of 17.4 kDa.
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Cat. No. : IL1A-5432P
Description : IL1A is a protein that in humans is encoded bythe IL1A gene. The protein encoded by this gene is a cytokine of theinterleukin-1 family. IL1A possesses a wide spectrum of metabolic,physiological, haematopoietic activities, and plays one of the central rolesin the regulation of the immune responses. It binds to the interleukin-1receptor. IL1A is produced mainly by activatedmacrophages, as well as neutrophils, epithelial cells, and endothelial cells.In general, Interleukin 1 is responsible for the production of inflammation,as well as the promotion of fever and sepsis.
Source : E. coli
Species : Pig
Form : Filtered through a0.22 micron sterile filter. Then lyophilized, carrier-free.
Molecular Weight : 17.4 kDa
Purity : >95% asdetermined by SDS-PAGE. Purified via sequential chromatography.
Endotoxin Level : <0.1 ng/µg ofprotein
Biological Activity : Determined by theD10S cell line proliferation assay with MTS detection. The ED50 is 0.02-0.04ng/ml.
Reconstitution : Centrifuge vialprior to opening. Lyophilized IL-1alpha may be reconstituted in water, orother appropriate buffered solutions to a concentration of 0.1-1.0 mg/ml.These stock solutions should be apportioned into working aliquots and storedat -20°C to -80°C (not a frost-free refrigerator). Further dilution should bemade in medium or buffered solution containing tissue culture grade carrierprotein, such as PBS with 0.1-1.0% BSA. Please note that the addition of anycarrier protein into this product may produce unwanted endotoxin. Thisdepends upon the particular application employed.
Storage : Lyophilized IL-1alpha should be stored at 2-4°C, preferably desiccated. Store reconstitutedIL-1 alpha at -20°C to -80°C (not in a frost-free refrigerator). When stored properly,IL-1 alpha is stable for greater than one year lyophilized and greater than 3months reconstituted. Avoid repeated freeze-thaw cycles.
OfficialSymbol : IL1A
SDS-PAGE:
Gene Name : IL1A interleukin 1, alpha [ Susscrofa ]
Synonyms : IL1A; interleukin 1,alpha; IL-1alpha; interleukin-1 alpha; IL-1 alpha; interleukin 1-alpha
Gene ID : 397094
mRNA Refseq : NM_214029
Protein Refseq : NP_999194
UniProt ID : P18430
Chromosome Location : 3q12-q13; 3
Pathway : Apoptosis;Cytokine-cytokine receptor interaction; Graft-versus-host disease;Hematopoietic cell lineage; Influenza A; Leishmaniasis; MAPK signalingpathway; Measles; Osteoclast differentiation; Pertussis; Prion diseases;Rheumatoid arthritis; Salmonella infection; Tuberculosis; Type I diabetesmellitus

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What methods are employed to dissect the intricate cross-talk between IL-1α and other cytokines, shedding light on its role in immune modulation? 11/02/2021

Cross-talk between IL-1α's and other cytokines is dissected through methods such as co-immunoprecipitation, ELISA, and cytokine profiling arrays.

What experimental approaches allow researchers to dissect the paradoxical roles of IL-1α as both a pro-inflammatory and tissue repair mediator in distinct contexts? 09/22/2021

IL-1α's paradoxical roles are dissected using conditional knockout mice, 3D culture systems, and molecular analyses to delineate context-specific effects.

How are cutting-edge techniques like single-cell RNA sequencing utilized to unravel the heterogeneity in IL-1α-responsive cell populations and their functional implications? 06/07/2021

Single-cell RNA sequencing uncovers heterogeneity in IL-1α-responsive cell populations and their roles, revealing distinct molecular profiles and functions.

How is the intricate web of IL-1α's pro-inflammatory signaling pathways decoded using advanced experimental techniques? 05/26/2021

IL-1α's intricate pro-inflammatory signaling pathways are decoded using advanced techniques like mass spectrometry, phosphoproteomics, and bioinformatics.

How are state-of-the-art genetic editing tools such as CRISPR-Cas9 applied to unravel IL-1α's precise contributions to inflammatory diseases? 04/06/2021

Genetic editing tools like CRISPR-Cas9 unveil IL-1α's contributions to diseases by generating knockout models and studying resulting phenotypic changes.

How do researchers manipulate in vitro co-culture systems to dissect the dynamic interplay between immune cells and stromal cells in IL-1α-mediated responses? 06/18/2020

In vitro co-culture systems are manipulated to study IL-1α-mediated responses, elucidating immune-stromal cell interactions via cytokine-specific neutralization.

What experimental strategies enable the identification of specific cellular receptors and downstream effectors responsible for IL-1α's diverse physiological effects? 03/19/2020

Specific cellular receptors and effectors influenced by IL-1α are identified through techniques like co-immunoprecipitation, ChIP-seq, and siRNA knockdown.

How are sophisticated imaging techniques like live-cell microscopy harnessed to capture the real-time dynamics of IL-1α release and its impact on neighboring cells? 12/02/2019

Live-cell microscopy captures real-time IL-1α release dynamics and its impact on neighboring cells, facilitated by fluorescent tagging and high-resolution imaging.

How is the quantitative relationship between IL-1α levels and disease progression established through longitudinal studies and statistical modeling? 07/15/2018

Longitudinal studies and statistical modeling establish the quantitative relationship between IL-1α levels and disease progression, offering predictive insights.

How do multi-omics analyses, including transcriptomics and proteomics, provide a holistic view of the network of molecules influenced by IL-1α signaling? 06/04/2018

Multi-omics analyses provide a holistic view of IL-1α signaling's impact, integrating transcriptomics, proteomics, and metabolomics data for comprehensive insights.

Customer Reviews (3)

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Reviews
04/08/2021

    High solubility.

    07/02/2020

      Good for intracellular staining.

      03/13/2019

        Effective in apoptosis assays.

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