Active Recombinant Human IL1A protein, His-tagged
|Product Overview :||Recombinant Human IL-1 alpha Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ser113-Ala271) of human IL-1 alpha/IL1A/IL1F1 (Accession #NP_000566.3) fused with a 6×His tag at the C-terminus.|
- Gene Information
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|Form :||Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.|
|Bio-activity :||Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IL1A at 5 μg/mL (100 μL/well) can bind recombinant Human IL1R2,the ED50 of Human IL1R2 is 2.18 μg/mL.|
|Molecular Mass :||21 kDa|
|Protein Length :||Ser113-Ala271|
|Endotoxin :||< 0.1 EU/μg of the protein by LAL method.|
|Purity :||> 95% by SDS-PAGE.|
|Storage :||Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
|Reconstitution :||Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.|
|Gene Name :||IL1A interleukin 1, alpha [ Homo sapiens ]|
|Official Symbol :||IL1A|
|Synonyms :||IL1A; interleukin 1, alpha; IL1; interleukin-1 alpha; hematopoietin 1; IL 1A; IL1 ALPHA; IL1F1; preinterleukin 1 alpha; pro interleukin 1 alpha; IL-1 alpha; hematopoietin-1; pro-interleukin-1-alpha; IL-1A; IL1-ALPHA;|
|Gene ID :||3552|
|mRNA Refseq :||NM_000575|
|Protein Refseq :||NP_000566|
|UniProt ID :||P01583|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
Cross-talk between IL-1α's and other cytokines is dissected through methods such as co-immunoprecipitation, ELISA, and cytokine profiling arrays.
IL-1α's paradoxical roles are dissected using conditional knockout mice, 3D culture systems, and molecular analyses to delineate context-specific effects.
Single-cell RNA sequencing uncovers heterogeneity in IL-1α-responsive cell populations and their roles, revealing distinct molecular profiles and functions.
IL-1α's intricate pro-inflammatory signaling pathways are decoded using advanced techniques like mass spectrometry, phosphoproteomics, and bioinformatics.
Genetic editing tools like CRISPR-Cas9 unveil IL-1α's contributions to diseases by generating knockout models and studying resulting phenotypic changes.
In vitro co-culture systems are manipulated to study IL-1α-mediated responses, elucidating immune-stromal cell interactions via cytokine-specific neutralization.
Specific cellular receptors and effectors influenced by IL-1α are identified through techniques like co-immunoprecipitation, ChIP-seq, and siRNA knockdown.
Live-cell microscopy captures real-time IL-1α release dynamics and its impact on neighboring cells, facilitated by fluorescent tagging and high-resolution imaging.
Longitudinal studies and statistical modeling establish the quantitative relationship between IL-1α levels and disease progression, offering predictive insights.
Multi-omics analyses provide a holistic view of IL-1α signaling's impact, integrating transcriptomics, proteomics, and metabolomics data for comprehensive insights.
Customer Reviews (3)Write a review
High solubility. -
Good for intracellular staining. -
Effective in apoptosis assays. -
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