Active Recombinant Rat Pdgfrb Protein, hFc-tagged

• Cat.No.: Pdgfrb-306R
• Product Overview: Recombinant Rat Pdgfrb Protein(Q05030) (Met1-Lys530) was expressed in HEK293, fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: hFc
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA.
  Immobilized mouse PDGF-B at 10μg/mL (100μL/well) can bind rat PDGFRB-Fc, the EC50 of rat PDGFRB-Fc is 0.02-0.2 μg/mL.
• Molecular Mass: The recombinant rat PDGFRB/Fc is a disulfide-linked homodimer. The reduced monomer comprises 740 amino acids and has a predicted molecular mass of 83.2 kDa. The apparent molecular mass of the protein is approximately 117 kDa in SDS-PAGE under reducing conditions.
• Protein length: Met1-Lys530
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Pdgfrb platelet derived growth factor receptor, beta polypeptide [ Rattus norvegicus ]
• Official Symbol: Pdgfrb
• Synonyms: PDGFRB; platelet derived growth factor receptor, beta polypeptide; platelet-derived growth factor receptor beta; PDGFR-beta; PDGF-R-beta; CD140 antigen-like family member B; platelet-derived growth factor receptor 1; beta platelet-derived growth factor receptor; Platelet-derived growth factor receptor, beta; beta-type platelet-derived growth factor receptor; platelet-derived growth factor receptor beta variant 1; PDGFR-1
• Gene ID: 24629
• mRNA Refseq: NM_031525
• Protein Refseq: NP_113713