| Description : |
CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymph centigradeytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. Upon ligand binding, 4-1BB is ass centigradeiated with the tumor necrosis factor receptor–ass centigradeiated factors (TRAFs), the adaptor protein which mediates downstream signaling events including the activation of NF-kappaB and cytokine production. 4-1BB signaling either by binding to 4-1BBL or by antibody ligation delivers signals for T-cell activation and growth, as well as mon centigradeyte proliferation and B-cell survival, and plays an important role in the amplification of T cell-mediated immune responses. In addition, CD137 and CD137L are expressed in different human primary tumor tissues, suggesting that they may influence the progression of tumors. Crosslinking of CD137 on activated T cells has shown promise in enhancing anti-tumor immune responses in murine models, and agonistic anti-CD137 antibodies are currently being tested in phase I clinical trials. |
| Source : |
HEK293 |
| Species : |
Rhesus |
| Tag : |
His |
| Predicted N Terminal : |
Leu 24 |
| Form : |
Lyophilized from sterile PBS, pH 7.4. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : |
Measured by its binding ability in a functional ELISA.Immobilized rhesus TNFRSF9-His at 10μg/mL (100μL/well) can bind human TNFSF9, the EC50 of human TNFSF9 is 10-60ng/mL. |
| Molecular Mass : |
The recombinant rhesus TNFRSF9 consists of 174 amino acids and predicts a molecular mass of 18.7 kDa. |
| Protein length : |
Met1-Gln186 |
| Endotoxin : |
< 1.0 EU per μg protein as determined by the LAL method. |
| Purity : |
> (40.7+53.4) % as determined by SDS-PAGE. |
| Storage : |
Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
| Official Symbol : |
TNFRSF9 |
| Synonyms : |
TNFRSF4;TNF receptor superfamily member 4;tumor necrosis factor receptor superfamily member 4;tumor necrosis factor receptor superfamily, member 4 |
