| Species : |
Streptomyces avidinii |
| Source : |
E.coli |
| Tag : |
Non |
| Protein Length : |
37-163 aa |
| Conjugation/Label : |
Azide |
| Description : |
Streptavidin-azide is an azide-modified streptavidin that is designed for site-specific conjugation by click chemistry reaction. The sole azido group is located at the c-terminus of streptavidin. It reacts very efficiently to Dibenzocyclooctyne (DBCO) or alkyne-modified molecules and forms a stable covalent bond. In addition, streptavidin-azide contains a long polyethylene glycol (PEG) spacer (76 atoms) to span the protein and azide. Such a design minimizes the interference between the biotin-binding site and the conjugated molecules. Hence, this innovative streptavidin-azide is extremely useful when random amine-mediated conjugation interferes with the function of the conjugate. In contrast, generally, modification and conjugation of streptavidin is performed on random amines provided by lysine residues and the n-terminal amino acid. Because streptavidin contains at least 5 amino groups, the amine-based conjugation is not site-specific and the products are heterogeneous with a low activity. However, our streptavidin-azide can generate a homogeneous conjugate. |
| Form : |
Liquid |
| Molecular Mass : |
14.5 kDa (Monomer) or 58.0 kDa (Tetramer) |
| Purity : |
≥ 95% by SDS-PAGE |
| Applications : |
In vitro research use only
Antibodies, oligonucleotides, enzymes (HRP, phosphatase), fluorescent dyes (Alexa, ATTO, FITC, R-PE, APC, PerCP), drugs, peptides, and solid supports can be modified by DBCO or alkyne crosslinkers. The modified biomolecules are attached to the c-terminus of Streptavidin-azide at a controllable ratio. In addition, the coupling is very efficient with high yields and purity. Moreover, the conjugates retain the biotin-binding activity. |
| Storage : |
At -20 centigrade. Avoid freeze/thaw cycle |
| Storage Buffer : |
Sodium phosphate buffer, pH7.2 |
| Concentration : |
> 1.0 mg/mL |
| Shipping : |
Cold packs |
