Recombinant Streptomyces avidinii Streptavidin, Tag Free, Azide Labeled

• Cat.No.: Streptavidin-13
• Product Overview: Recombinant Streptomyces avidinii Streptavidin without tag was expressed in E. coli.

Specification

• Species: Streptomyces avidinii
• Source: E.coli
• Tag: Non
• Protein Length: 37-163 aa
• Conjugation/Label: Azide
• Description: Streptavidin-azide is an azide-modified streptavidin that is designed for site-specific conjugation by click chemistry reaction. The sole azido group is located at the c-terminus of streptavidin. It reacts very efficiently to Dibenzocyclooctyne (DBCO) or alkyne-modified molecules and forms a stable covalent bond. In addition, streptavidin-azide contains a long polyethylene glycol (PEG) spacer (76 atoms) to span the protein and azide. Such a design minimizes the interference between the biotin-binding site and the conjugated molecules. Hence, this innovative streptavidin-azide is extremely useful when random amine-mediated conjugation interferes with the function of the conjugate. In contrast, generally, modification and conjugation of streptavidin is performed on random amines provided by lysine residues and the n-terminal amino acid. Because streptavidin contains at least 5 amino groups, the amine-based conjugation is not site-specific and the products are heterogeneous with a low activity. However, our streptavidin-azide can generate a homogeneous conjugate.
• Form: Liquid
• Molecular Mass: 14.5 kDa (Monomer) or 58.0 kDa (Tetramer)
• Biotin binding Site: 0
• Purity: ≥ 95% by SDS-PAGE
• Applications: In vitro research use only Antibodies, oligonucleotides, enzymes (HRP, phosphatase), fluorescent dyes (Alexa, ATTO, FITC, R-PE, APC, PerCP), drugs, peptides, and solid supports can be modified by DBCO or alkyne crosslinkers. The modified biomolecules are attached to the c-terminus of Streptavidin-azide at a controllable ratio. In addition, the coupling is very efficient with high yields and purity. Moreover, the conjugates retain the biotin-binding activity.
• Storage: At -20 centigrade. Avoid freeze/thaw cycle
• Storage Buffer: Sodium phosphate buffer, pH7.2
• Concentration: > 1.0 mg/mL
• Shipping: Cold packs

Gene Information

• Official Symbol: Streptavidin
• Synonyms: Streptavidin
• UniProt ID: P22629