CD48

  • Official Full Name

    CD48 CD48 molecule

  • Overview

    CD48 is a glycosylphosphatidylinositol-anchored protein belonging to the CD2 subfamily. It is expressed mainly on hematopoietic cells and exists in both membrane-associated and soluble forms. It is a low-affinity ligand for CD2 and is implicated as an important costimulatory molecule in lymphocyte activation. Indeed, CD48 has been described as facilitating cell adhesion, innate responses to bacterial infection, and graft rejection. In addition, CD48 is a high-affinity ligand for 2B4. CD482B4 interactions can modulate T cell, B cell, and natural killer (NK) cell functions and cross-talk.The absence of functional 2B4 CD48 interactions impairs NK cell cytotoxic response and IFN-release on tumor target exposure. Furthermore, activated NK cells significantly increase the CD3-dependent proliferation of CD8+ and CD4+ T cells by a 2B4CD48-dependent mechanism.
  • Synonyms

    CD48; CD48 molecule; BCM1, CD48 antigen (B cell membrane protein) , CD48 molecule; CD48 antigen; BLAST; hCD48; mCD48; SLAMF2; TCT.1; BCM1 surface antigen; leukocyte antigen MEM-102; B-lymphocyte activation marker BLAST-1; CD48 antigen (B-cell membrane protein); BCM1; BLAST1; MEM-102;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Antibody
  • GMP Proteins
  • Protein Pre-coupled Magnetic Beads
  • Human
  • Mouse
  • Rat
  • CHO
  • CHO cells
  • E.coli
  • HEK293
  • Human Cell
  • Human Cells
  • In Vitro Cell Free System
  • Mammalian Cell
  • Mammalian cells
  • Rabbit
  • Wheat Germ
  • Yeast
  • C
  • Fc
  • hFc
  • His
  • Avi
  • Fc|His
  • His(C
  • ter)
  • GST
  • His (Fc)
  • His|T7
  • human|IgG1|Fc
  • Mouse|IgG2a|Fc
  • N/A
  • N
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human CD48-680H Active Recombinant Human CD48, Fc-tagged, Biotinylated Human Cell Fc
Human CD48-3255H Active Recombinant Human CD48 protein(Met1-Ser220), His&Avi-tagged, Biotinylated HEK293 C-His&Avi Met1-Ser220
Human CD48-051H Active Recombinant Human CD48 protein, His/Avi-tagged, Biotinylated CHO cells His/Avi Gln27-Ser220
Human CD48-10960H Recombinant Human CD48 Protein, GST-tagged E.coli GST 27-220 aa
Human CD48-0828H Recombinant Human CD48 Protein, GST-Tagged Wheat Germ GST
Human CD48-234H Recombinant Human CD48 protein, Fc-tagged HEK293 human/IgG1/Fc Gln27-Ser220
Human CD48-654H Recombinant Human CD48 protein, His-tagged HEK293 His Gln27-Ser220
Human CD48-1871H Recombinant Human CD48 protein, His & T7-tagged E.coli His/T7 Gln27~Thr243 (Accession # P09326)
Human CD48-719H Recombinant Human CD48 Protein CHO N/A
Human CD48-838H Recombinant Human CD48 Protein, Fc/His-tagged HEK293 Fc/His
Human CD48-26635TH Recombinant Human CD48 Wheat Germ N/A 169 amino acids
Human CD48-252H Active Recombinant Human CD48 protein, hFc-tagged HEK293 hFc Met1-Ser220
Human CD48-3044HCL Recombinant Human CD48 cell lysate Human Cell N/A
Human CD48-646H Recombinant Human CD48 Protein HEK293 243
Human CD48-357H Recombinant Human CD48 protein, Mouse IgG2a Fc-tagged, low endotoxin HEK293 Mouse/IgG2a/Fc 194
Human CD48-654HAF488 Recombinant Human CD48 Protein, His-tagged, Alexa Fluor 488 conjugated HEK293 His Gln27-Ser220
Human CD48-5369H Recombinant Human CD48 protein, hFc-tagged Yeast hFc 27-220aa
Human CD48-200H Recombinant Human CD48 Protein, C-His-tagged E.coli C-His
Human CD48-252HF Recombinant Human CD48 Protein, Fc-tagged, FITC conjugated HEK293 Fc Met1-Ser220, 432
Human CD48-252HAF647 Recombinant Human CD48 Protein, Fc-tagged, Alexa Fluor 647 conjugated HEK293 Fc Met1-Ser220, 432
Human CD48-63HF Recombinant Full Length Human CD48 Protein In Vitro Cell Free System 169 amino acids
Human CD48-654HAF647 Recombinant Human CD48 Protein, His-tagged, Alexa Fluor 647 conjugated HEK293 His Gln27-Ser220
Human CD48-654HF Recombinant Human CD48 Protein, His-tagged, FITC conjugated HEK293 His Gln27-Ser220
Human CD48-2998HF Recombinant Full Length Human CD48 Protein, GST-tagged In Vitro Cell Free System GST 169 amino acids
Human CD48-34H Recombinant Human CD48 Protein (ECD), Fc-His-tagged(C-ter) HEK293 Fc-His(C-ter) Gln27-Ser220
Human CD48-2638H Active Recombinant Human CD48 protein, hFc&His-tagged HEK293 hFc&His Gln27-Ser220
Human CD48-509H Recombinant Human CD48 Protein (Gln27-Ser220), C-hFc-tagged HEK293 C-hFc Gln27-Ser220
Human CD48-0263H Active Recombinant Human CD48 protein, hFc&Avi-tagged, Biotinylated HEK293 hFc&Avi Met1-Ser220
Human CD48-35H Recombinant Human CD48 Protein (ECD), His-tagged(C-ter) HEK293 His(C-ter) Gln27-Ser220
Human CD48-1416H Recombinant Human CD48 Protein (Gln27-Ser220), N-His tagged E.coli N-His Gln27-Ser220
Human CD48-2179H Active Recombinant Human CD48 protein, His-tagged HEK293 His Met1-Ser220
Human CD48-5356H Recombinant Human CD48 Protein (Met1-Ser220), C-His tagged Mammalian cells C-His Met1-Ser220
Human CAB11502RH Rabbit Anti-Human CD48 Molecule Monoclonal Antibody Rabbit N/A
Human CD48-5357H Recombinant Human CD48 Protein (Met1-Ser220), C-Fc tagged Mammalian cells C-Fc Met1-Ser220
Human CD48-252HAF555 Recombinant Human CD48 Protein, Fc-tagged, Alexa Fluor 555 conjugated HEK293 Fc Met1-Ser220, 432
Human CD48-2042H Recombinant Human CD48 Protein, Fc/His-tagged HEK293 Fc/His Gln27-Ser220
Human CD48-654HAF555 Recombinant Human CD48 Protein, His-tagged, Alexa Fluor 555 conjugated HEK293 His Gln27-Ser220
Human CD48-204HG Active GMP Recombinant Human CD48 protein HEK293 DNA sequence encoding Human CD48(GenBank: AAH16182.1) was expressed with His tag at the C-terminal.
Human CD48-151H Recombinant Human CD48 Protein, His-tagged Human Cells His
Human CD48-252HAF488 Recombinant Human CD48 Protein, Fc-tagged, Alexa Fluor 488 conjugated HEK293 Fc Met1-Ser220, 432
Mouse CD48-867M Active Recombinant Mouse CD48 Protein, His-tagged HEK293 His
Mouse Cd48-459M Active Recombinant Mouse Cd48, His-tagged Mammalian cells His
Mouse CD48-867MAF555 Active Recombinant Mouse CD48 Protein, His-tagged, Alexa Fluor 555 conjugated HEK293 His 205
Mouse CD48-867MAF488 Active Recombinant Mouse CD48 Protein, His-tagged, Alexa Fluor 488 conjugated HEK293 His 205
Mouse CD48-867MF Active Recombinant Mouse CD48 Protein, His-tagged, FITC conjugated HEK293 His 205
Mouse CD48-867MAF647 Active Recombinant Mouse CD48 Protein, His-tagged, Alexa Fluor 647 conjugated HEK293 His 205
Mouse Cd48-1872M Recombinant Mouse Cd48 protein, His & T7-tagged E.coli His/T7 Phe23~Ser217 (Accession # P18181)
Mouse CD48-720M Recombinant Mouse CD48 Protein CHO N/A
Mouse Cd48-3293M Recombinant Mouse CD48 Antigen, His-tagged Human Cell His
Mouse Cd48-486M Recombinant Mouse Cd48, Fc-tagged Human Cell Fc Met1-Arg216
Mouse CD48-2468MCL Recombinant Mouse CD48 cell lysate Human Cell N/A
Mouse CD48-867MB Recombinant Mouse CD48 protein, His-tagged, Biotinylated HEK293 His Met1-Arg216
Mouse Cd48-486MAF488 Recombinant Mouse Cd48 Protein, Fc-tagged, Alexa Fluor 488 conjugated HEK293 Fc Met1-Arg216, 432
Mouse Cd48-486MAF555 Recombinant Mouse Cd48 Protein, Fc-tagged, Alexa Fluor 555 conjugated HEK293 Fc Met1-Arg216, 432
Mouse Cd48-486MAF647 Recombinant Mouse Cd48 Protein, Fc-tagged, Alexa Fluor 647 conjugated HEK293 Fc Met1-Arg216, 432
Mouse Cd48-1464M Recombinant Mouse Cd48 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse Cd48-486MF Recombinant Mouse Cd48 Protein, Fc-tagged, FITC conjugated HEK293 Fc Met1-Arg216, 432
Mouse Cd48-1464M-B Recombinant Mouse Cd48 Protein Pre-coupled Magnetic Beads HEK293
Mouse Cd48-6807M Recombinant Mouse Cd48 Protein (Phe23-Arg216), C-His tagged Mammalian cells C-His Phe23-Arg216
Rat Cd48-8763R Recombinant Rat Cd48 protein, His-tagged HEK293 His Met1-Arg216
Rat Cd48-8762R Recombinant Rat Cd48 protein(Met1-Arg216), hFc-tagged HEK293 C-hFc Met1-Arg216
Rat CD48-1256R Recombinant Rat CD48 Protein Mammalian Cell His
Rat Cd48-1873R Recombinant Rat Cd48 protein, His & T7-tagged E.coli His/T7 Phe23~Ala240 (Accession # P10252)
Rat CD48-1258RCL Recombinant Rat CD48 cell lysate Human Cell N/A
Rat CD48-914R-B Recombinant Rat CD48 Protein Pre-coupled Magnetic Beads HEK293
Rat Cd48-8762RAF555 Recombinant Rat Cd48 Protein, Fc-tagged, Alexa Fluor 555 conjugated HEK293 Fc 435
Rat CD48-914R Recombinant Rat CD48 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rat Cd48-8763RAF488 Recombinant Rat Cd48 Protein, His-tagged, Alexa Fluor 488 conjugated HEK293 His 205
Rat Cd48-8762RAF488 Recombinant Rat Cd48 Protein, Fc-tagged, Alexa Fluor 488 conjugated HEK293 Fc 435
Rat Cd48-8763RAF555 Recombinant Rat Cd48 Protein, His-tagged, Alexa Fluor 555 conjugated HEK293 His 205
Rat Cd48-8762RAF647 Recombinant Rat Cd48 Protein, Fc-tagged, Alexa Fluor 647 conjugated HEK293 Fc 435
Rat Cd48-8763RAF647 Recombinant Rat Cd48 Protein, His-tagged, Alexa Fluor 647 conjugated HEK293 His 205
Rat Cd48-8762RF Recombinant Rat Cd48 Protein, Fc-tagged, FITC conjugated HEK293 Fc 435
Rat Cd48-8763RF Recombinant Rat Cd48 Protein, His-tagged, FITC conjugated HEK293 His 205
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource
CD48-9.jpg

Fig1. NK cell education in XLP1 as compared to healthy controls. (Raffaella Meazza, 2017)

What is CD48 protein?

CD48 (CD48 molecule) gene is a protein coding gene which situated on the long arm of chromosome 1 at locus 1q23. CD48 is a glycosylphosphatidylinositol-anchored protein belonging to the CD2 subfamily. It is expressed mainly on hematopoietic cells and exists in both membrane-associated and soluble forms. It is a low-affinity ligand for CD2 and is implicated as an important costimulatory molecule in lymphocyte activation. Indeed, CD48 has been described as facilitating cell adhesion, innate responses to bacterial infection, and graft rejection. In addition, CD48 is a high-affinity ligand for 2B4. The CD48 protein is consisted of 243 amino acids and its molecular mass is approximately 27.7 kDa.

What is the function of CD48 protein?

CD48, by binding to its ligand CD2, is involved in the recognition and activation of T cells, thus playing a key role in adaptive immune responses. Binding of CD48 to CD2 is also involved in the regulation of natural killer (NK) cells, influencing their mediated cytotoxic activities and thus also playing a role in the innate immune response. In interactions between lymphocytes, dendritic cells, macrophages, etc., CD48 helps transmit signals that guide cell migration and tissue localization, as well as subsequent immune responses. In some cases, CD48 may be involved in maintaining autoimmune tolerance, preventing the immune system from mistakenly attacking the body's tissues.

CD48 Related Signaling Pathway

The binding of CD48 to CD2 is one of the key steps in T cell activation. This process triggers a series of signaling events, including the activation of protein kinase C (PKC), the opening of calcium channels, and an increase in calcium ion concentration. These signals ultimately lead to the activation of transcription factors such as NF-kB and AP-1, which trigger changes in gene expression and regulation of cell function. In natural killer (NK) cells, the interaction of CD48 with CD2 can also regulate its recognition and killing activity against target cells. This process involves a variety of signaling molecules and pathways, such as SRC-family kinases and phosphatidylinositol 3-kinase (PI3K)/Akt pathway.

CD48 Related Diseases

CD48 plays a key role in the activation, proliferation and cytotoxicity of T and NK cells. Therefore, its abnormalities may be associated with autoimmune diseases, allergic reactions, and immune deficiencies. Abnormalities in CD48 may also be associated with other diseases, such as tumors, cardiovascular diseases, inflammatory diseases, and infectious diseases

Bioapplications of CD48

In some disease states, such as certain types of cancer and viral infections, the expression pattern of CD48 may change. Therefore, it can be used as a biomarker for these diseases. At the same time, monoclonal antibody therapy against CD48 is also being investigated for the treatment of certain blood diseases and solid tumors. Because of the important role of CD48 in immune response, it may also be involved in immune rejection after organ transplantation. Therefore, its study may help improve the success rate of transplantation.

High Purity

High Bioactivity

CD48-2.jpg

Fig2. Activity Data. (CD48-051H)

Case Study 1: Katalin Kis-Toth, 2014

Signaling lymphocyte activation molecule family (SLAMF)2/CD48 is a coactivator and adhesion molecule on cells with hematopoietic origin. It ligates mainly SLAMF4 on effector/memory CD8(+) T cells and NK cells, suggesting a potential role during viral infection, with SLAMF2 acting as a ligand to activate SLAMF4-bearing cells. The ability of SLAMF2 to signal on its own after it is engaged and the functional consequences are largely unknown. The researchers found that cytosolic DNA-activated dendritic cells (DCs) upregulate the expression of SLAMF2 molecules. Using anti-SLAMF2 Ab and SLAMF4 recombinant protein, they found that SLAMF2 engagement activates immature DCs and, more interestingly, prolongs the survival of DNA-activated DCs by inhibiting IFN-β production and IFN-β-induced apoptosis and promotes the production of the granzyme B inhibitor protease inhibitor-9. Thus, SLAMF2 can serve as a survival molecule for DNA-activated DCs during their interaction with SLAMF4-expressing cytotoxic T cells. Based on these results, they propose that SLAMF2 engagement regulates adaptive immune responses by providing longer access of putative APCs to virus-specific effector T cells by prolonging the time frame of effective stimulation.

CD48-3.jpg

Fig1. Histograms show the distribution of SLAMF2 molecules on the surface of CD1a+ and CD1a− IDC and DNA-DC populations.

CD48-4.jpg
Fig2. SLAMF2 engagement rescues mature dendritic cells from cell death by inhibiting the production of IFNβ and the expression of caspase-4.

Case Study 2: Zhaowei Wang, 2021

A better understanding of the molecular mechanisms that manifest in the immunosuppressive tumor microenvironment (TME) is crucial for developing more efficacious immunotherapies for hepatocellular carcinoma (HCC), which has a poor response to current immunotherapies. Regulatory T (Treg) cells are key mediators of HCC-associated immunosuppression. The researchers investigated the selective mechanism exploited by HCC that lead to Treg cells expansion and to find more efficacious immunotherapies.

They used mass cytometry (CyTOF) and orthotopic cancer mouse models to analyze overall immunological changes after growth differentiation factor 15 (GDF15) gene ablation in HCC. They used flow cytometry, coimmunoprecipitation, RNA sequencing, mass spectrum, chromatin immunoprecipitation and Gdf15-/-, OT-I and GFP transgenic mice to demonstrate the effects of GDF15 on Treg cells and related molecular mechanism. The results showed GDF15 promotes the generation of peripherally derived inducible Treg (iTreg) cells and enhances the suppressive function of natural Treg (nTreg) cells by interacting with a previously unrecognized receptor CD48 on T cells and thus downregulates STUB1, an E3 ligase that mediates forkhead box P3 (FOXP3) protein degradation.

CD48-5.jpg

Fig3. Coimmunoprecipitation (CoIP) analysis of the interaction between GDF15 and CD48.

CD48-6.jpg
Fig4. The interaction of GDF15 with purified CD48, as measured by surface plasmon resonance (SPR) spectroscopy.
CD48-7.jpg

Fig1. CD48 on NK cells and 2B4 on macrophages suppress phagocytosis of NK cells in MCMV infection. (Rui Li, 2024)

CD48-8.jpg

Fig2. Macrophage–NK cell interactions. Diagram summarizes the cellular pathways that regulate the cross talk between human NK cells and M1 macrophages. (Irene Mattiola, 2015)

CD48 involved in several pathways and played different roles in them. We selected most pathways CD48 participated on our site, such as Natural killer cell mediated cytotoxicity, which may be useful for your reference. Also, other proteins which involved in the same pathway with CD48 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Natural killer cell mediated cytotoxicityULBP1;TNFSF10;PPP3CB;IFNA13;IFNG;MICA;HLA-A;MAP2K2;PPP3R1

CD48 has several biochemical functions, for example, protein binding. Some of the functions are cooperated with other proteins, some of the functions could acted by CD48 itself. We selected most functions CD48 had, and list some proteins which have the same functions with CD48. You can find most of the proteins on our site.

Function Related Protein
protein bindingTRIM24;ZC3HC1;JIP1;LRRC25;FGF21;BAG1;DEFB121;GPR158;KCNRG

CD48 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with CD48 here. Most of them are supplied by our site. Hope this information will be useful for your research of CD48.

CD244; EEF1D; yapA; BXDC2

Gene Family

Immunoglobulins

Research Area

Related articles

Meazza, R; Tuberosa, C; et al. XLP1 inhibitory effect by 2B4 does not affect DNAM-1 and NKG2D activating pathways in NK cells. EUROPEAN JOURNAL OF IMMUNOLOGY 44:1526-1534(2014).
Hashmi, FK; Cail, R; et al. Adaptation of WM-68 Hybridoma Cell-line in Minimal Serum and Serum Free Culture Conditions. PAKISTAN JOURNAL OF ZOOLOGY 46:355-362(2014).
  • Q&As
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Q&As (6)

Ask a question
Could the expression level of CD48 protein be modulated to treat disease? 11/02/2022

There is currently no treatment that directly regulates CD48 protein expression levels. However, by regulating the function of the immune system, the expression level of CD48 protein can be indirectly affected to treat some diseases.

How does the expression level of CD48 protein relate to the prognosis of tumors? 10/06/2022

Expression level of CD48 protein is associated with the prognosis of certain tumors. In some cancers, high levels of CD48 protein expression may predict a poor prognosis.

How does CD48 protein relate to the immune system? 04/16/2022

CD48 protein is an important molecule in the immune system that is involved in the activation and differentiation of lymphocytes and phagocytes, as well as inflammation and tissue repair. Its expression levels may affect the function of the immune system.

Does the level of CD48 protein expression correlate with vaccine efficacy? 06/09/2020

Studies have shown that the expression level of CD48 protein may affect the effectiveness of vaccination. In some cases, high levels of CD48 protein expression may suppress the immune response, thus affecting the effectiveness of vaccination.

Can CD48 protein be used as a biomarker for tumors? 03/26/2020

Yes, the CD48 protein can be used as a biomarker for some tumors. Studies have shown that in some cancers, high levels of CD48 protein expression may predict tumor growth and metastasis.

How does the expression level of CD48 protein relate to the occurrence and progression of the disease? 12/02/2019

Studies have shown that the expression level of CD48 protein may be associated with the occurrence and progression of certain diseases. For example, in some cancers, the expression level of the CD48 protein may affect the growth and metastasis of the tumor.

Customer Reviews (3)

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Reviews
09/25/2022

    The anti-interference ability in Western blot is very strong, which can effectively resist the influence of endogenous interference factors.

    05/14/2021

      High safety, high expression level, ideal for laboratory and practical applications.

      02/18/2021

        Low preparation cost, high cost performance, saving funds, very suitable for our research.

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