Recombinant Full Length Human JAK1 Protein, C-Flag-tagged

• Cat.No.: JAK1-151HFL
• Product Overview: Recombinant Full Length Human JAK1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta, interferon-gamma, and cytokine signal transduction. This gene plays a crucial role in effecting the expression of genes that mediate inflammation, epithelial remodeling, and metastatic cancer progression. This gene is a key component of the interleukin-6 (IL-6)/JAK1/STAT3 immune and inflammation response and is a therapeutic target for alleviating cytokine storms. The kinase activity of this gene is directly inhibited by the suppressor of cytokine signalling 1 (SOCS1) protein. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 133.1 kDa
• AA Sequence: MQYLNIKEDCNAMAFCAKMRSSKKT EVNLEAPEPGVEVIFYLSDREPLRL GSGEYTAEELCIRAAQACRI SPLCHNLFALYDENTKLWYAPNRTI TVDDKMSLRLHYRMRFYFTNWHGTN DNEQSVWRHSPKKQKNGYEK KKIPDATPLLDASSLEYLFAQGQYD LVKCLAPIRDPKTEQDGHDIENECL GMAVLAISHYAMMKKMQLPE LPKDISYKRYIPETLNKSIRQRNLL TRMRINNVFKDFLKEFNNKTICDSS VSTHDLKVKYLATLETLTKH YGAEIFETSMLLISSENEMNWFHSN DGGNVLYYEVMVTGNLGIQWRHKPN VVSVEKEKNKLKRKKLENKH KKDEEKNKIREEWNNFSYFPEITHI VIKESVVSINKQDNKKMELKLSSHE EALSFVSLVDGYFRLTADAH HYLCTDVAPPLIVHNIQNGCHGPIC TEYAINKLRQEGSEEGMYVLRWSCT DFDNILMTVTCFEKSEQVQG AQKQFKNFQIEVQKGRYSLHGSDRS FPSLGDLMSHLKKQILRTDNISFML KRCCQPKPREISNLLVATKK AQEWQPVYPMSQLSFDRILKKDLVQ GEHLGRGTRTHIYSGTLMDYKDDEG TSEEKKIKVILKVLDPSHRD ISLAFFEAASMMRQVSHKHIVYLYG VCVRDVENIMVEEFVEGGPLDLFMH RKSDVLTTPWKFKVAKQLAS ALSYLEDKDLVHGNVCTKNLLLARE GIDSECGPFIKLSDPGIPITVLSRQ ECIERIPWIAPECVEDSKNL SVAADKWSFGTTLWEICYNGEIPLK DKTLIEKERFYESRCRPVTPSCKEL ADLMTRCMNYDPNQRPFFRA IMRDINKLEEQNPDIVSEKKPATEV DPTHFEKRFLKRIRDLGEGHFGKVE LCRYDPEGDNTGEQVAVKSL KPESGGNHIADLKKEIEILRNLYHE NIVKYKGICTEDGGNGIKLIMEFLP SGSLKEYLPKNKNKINLKQQ LKYAVQICKGMDYLGSRQYVHRDLA ARNVLVESEHQVKIGDFGLTKAIET DKEYYTVKDDRDSPVFWYAP ECLMQSKFYIASDVWSFGVTLHELL TYCDSDSSPMALFLKMIGPTHGQMT VTRLVNTLKEGKRLPCPPNC
  PDEVYQLMRKCWEFQPSNRTSFQNL IEGFEALLKTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Jak-STAT signaling pathway, Pancreatic cancer, Pathways in cancer

Gene Information

• Gene Name: JAK1 Janus kinase 1 [ Homo sapiens (human) ]
• Official Symbol: JAK1
• Synonyms: JTK3; AIIDE; JAK1A; JAK1B
• Gene ID: 3716
• mRNA Refseq: NM_002227.4
• Protein Refseq: NP_002218.2
• MIM: 147795
• UniProt ID: P23458