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MMP13

  • Official Full Name

    matrix metallopeptidase 13 (collagenase 3)

  • Overview

    Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]
  • Synonyms

    MMP13; matrix metallopeptidase 13 (collagenase 3); CLG3; MANDP1; MMP-13; collagenase 3; matrix metalloproteinase 13 (collagenase 3);

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Cattle
  • Chicken
  • Dog
  • Human
  • Mouse
  • Pig
  • Rabbit
  • Rat
  • E.coli
  • HEK293
  • HEK293F
  • In Vitro Cell Free System
  • Mammalian Cell
  • Mammalian cells
  • Sf9 Insect Cell
  • Spodoptera Frugiperda
  • Wheat Germ
  • C
  • His
  • GST
  • His (Fc)
  • Avi
  • His(C
  • ter)
  • His|SUMO
  • His|T7
  • SUMO
  • N/A
  • N
  • No tag
  • T7|His
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human MMP13-810H Active Recombinant Human MMP13, His-tagged Sf9 Insect Cell His
Human MMP13-159H Active Recombinant Human MMP13 protein E.coli N/A
Human MMP13-27H Active Recombinant Human MMP13 protein E.coli N/A
Human MMP13-01H Active Recombinant Human MMP13 (F175D) Protein E.coli 104-268
Human MMP13-1018H Recombinant Human MMP13 E.coli N/A
Human MMP13-5044H Recombinant Human MMP13, His-tagged E.coli His
Human MMP13-43H Active Recombinant Human MMP-13, activated Spodoptera Frugiperda N/A
Human MMP13-443H Recombinant Human matrix metallopeptidase 13 (collagenase 3), His-tagged E.coli His
Human MMP13-92H Recombinant Human MMP13 protein, T7/His-tagged E.coli T7/His
Human MMP13-411H Recombinant Human Matrix Metallopeptidase 13 (collagenase 3) Mammalian cells N/A
Human MMP13-729H Recombinant Human MMP13 protein, His-tagged E.coli His Glu103~Leu290
Human MMP13-42H Recombinant Human MMP-13 Spodoptera Frugiperda N/A
Human MMP13-4280HCL Recombinant Human MMP13 293 Cell Lysate HEK293 N/A
Human MMP13-0832H Recombinant Human MMP13 Protein (E103-N274), Tag Free E.coli No tag E103-N274
Human MMP13-5420H Active Recombinant Human MMP13 Protein E.coli N/A
Human MMP13-4451H-B Recombinant Human MMP13 Protein Pre-coupled Magnetic Beads HEK293
Human MMP13-3231H Recombinant Human MMP13 protein, His-SUMO-tagged E.coli His-SUMO 104-471aa
Human MMP13-4575H Recombinant Human MMP13 Protein (Leu20-Cys471), N-His tagged E.coli N-His Leu20-Cys471
Human MMP13-5421H Recombinant Human MMP13 Protein, GST-tagged Wheat Germ GST
Human MMP13-0833H Recombinant Human MMP13 Protein (E103-N274), His tagged E.coli His E103-N274
Human MMP13-2448H Recombinant Human MMP13 Protein, His-tagged E.coli N-His Leu290-Ile468
Human MMP13-2765H Recombinant Human MMP13 protein(361-430 aa), C-His-tagged E.coli C-His 361-430 aa
Human MMP13-6454HF Recombinant Full Length Human MMP13 Protein, GST-tagged In Vitro Cell Free System GST 471 amino acids
Human MMP13-239H Recombinant Active Human MMP13 Protein, His-tagged(C-ter) HEK293 His(C-ter) Leu20-Cys471
Human MMP13-4451H Recombinant Human MMP13 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse Mmp13-731M Recombinant Mouse Mmp13 protein, His-tagged E.coli His Val66~Gly238 (Accession# P33435)
Mouse MMP13-9908M Recombinant Mouse MMP13 Protein Mammalian Cell His
Mouse Mmp13-3232M Recombinant Mouse Mmp13 protein, His-tagged E.coli His 105-472aa
Mouse Mmp13-1750M Recombinant Mouse Mmp13 protein, His-tagged E.coli His Ala98~Pro275
Mouse MMP13-5598M-B Recombinant Mouse MMP13 Protein Pre-coupled Magnetic Beads HEK293
Mouse MMP13-5598M Recombinant Mouse MMP13 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rat Mmp13-734R Recombinant Rat Mmp13 protein, His-tagged E.coli His Arg62~His217 (Accession# P23097)
Rat Mmp13-506R Recombinant Rat Mmp13 protein, His-tagged HEK293F His Leu14~Cys466
Rabbit MMP13-736R Recombinant Rabbit MMP13 protein, His-tagged E.coli His Glu70~Thr245 (Accession# O62806)
Dog MMP13-2045D Recombinant Dog MMP13 Protein (28-478 aa), His-SUMO-tagged E.coli His/SUMO 28-478 aa
Cattle MMP13-1119C Recombinant Cattle MMP13 Protein, His-tagged E.coli N-His Tyr104-Glu319
Pig MMP13-733P Recombinant Pig MMP13 protein, His & T7-tagged E.coli His/T7 Asn123~Glu270 (Accession# O77631)
Chicken MMP13-5521C Recombinant Chicken MMP13 Mammalian Cell His
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource

What is mmp13 protein?

MMP13 is mainly produced by chondrocytes, cells within cartilage, and plays an important role in cartilage remodeling and breakdown during development and growth. It is involved in endochondral ossification, the process by which bones are formed from cartilage templates. MMP13 action breaks down cartilage to allow its replacement with bone. Increased MMP13 expression/activity has been implicated in diseases involving cartilage degradation like osteoarthritis. It is a Marker of disease progression.MMP13 can also degrade other matrix proteins apart from collagens like aggrecan and pericellular matrix. Its activity is regulated at multiple levels including transcription, activation of zymogen, and inhibition by tissue inhibitors of metalloproteinases (TIMPs).

What are the functions of MMP13 protein?

Cartilage breakdown: MMP13 is specialized in degrading type II collagen, which is the most abundant collagen in cartilage. It breaks down the collagen network in cartilage.

Bone development: MMP13 plays an important role in endochondral ossification by degrading cartilage templates to allow their replacement with bone. This allows for proper bone growth and modeling.

Tissue remodeling: Along with collagen degradation, MMP13 can also cleave other extracellular matrix proteins like aggrecan. This allows for tissue remodeling during development, growth and wound healing.

Arthritis: Increased MMP13 expression and activity leads to excess cartilage breakdown in arthritic conditions like osteoarthritis. It contributes to disease pathogenesis.

MMP13 related signaling pathway

MAPK pathway: MMP13 expression is regulated by the MAPK signaling cascade, especially the ERK1/2 and p38 pathways. Factors like IL-1β and TGF-β induce MMP13 via MAPKs.

NF-κB pathway: Inflammatory cytokines like IL-1β activate NF-κB signaling to upregulate MMP13 transcription. NF-κB binds to MMP13 promoter region.

Wnt/β-catenin pathway: Wnt ligands induce β-catenin nuclear translocation and transcriptional activation of MMP13. This pathway mediates MMP13 expression in arthritis.

Notch pathway: Notch intracellular domain binds to RBP-Jκ and induces MMP13 expression in chondrocytes and osteoblasts during skeletal development.

MMP13 Related Diseases

  •  Osteoarthritis: Increased MMP13 expression contributes to cartilage degradation in osteoarthritis. It is a marker for disease progression.
  •  Rheumatoid arthritis: MMP13 levels correlate with joint damage in RA. Inhibiting it may help slow disease progression.
  •  Developmental skeletal diseases: Mutations affecting MMP13 cause polymorphic chondrodysplasias due to impaired endochondral ossification.
  •  Cancer metastasis: Many cancers like gastric, breast and prostate show elevated MMP13. It promotes tumor cell invasion and metastasis.
  •  Periodontitis: MMP13 levels increase in gingival crevicular fluid of periodontitis patients, destroying ligament and bone tissues.
  •  Disease biomarker: MMP13 levels can serve as an indicator of disease progression in osteoarthritis, rheumatoid arthritis and cancer metastasis.
  •  Drug target: Developing selective MMP13 inhibitors is a promising approach for arthritis and cancer therapeutic intervention.
  •  Tissue engineering: Regulations of MMP13 activity could promote cartilage regeneration for treating osteoarthritis and other joint disorders.
  •  Disease biomarker: MMP13 levels can serve as an indicator of disease progression in osteoarthritis, rheumatoid arthritis and cancer metastasis.
  •  Drug target: Developing selective MMP13 inhibitors is a promising approach for arthritis and cancer therapeutic intervention.
  •  Tissue engineering: Regulations of MMP13 activity could promote cartilage regeneration for treating osteoarthritis and other joint disorders.

High Purity

SDS-PAGE MMP13-733P.jpg

Fig1. SDS-PAGE (Cat. No.: MMP13-733P)

The expression of MMP13.jpg

(Jing Ma, 2014)

Fig2. The expression of MMP13 mRNA and protein was gradually and significantly increased from 1 to 7 days after the alkali burns.

The inhibitory effect of 444283 on MMP13 activity.jpg

(Jing Ma, 2014)

Fig3.The inhibitory effect of 444283 on MMP13 activity in alkali-burned rat corneas at day 7. Fifty μM of 444283 inhibited approximately 30% of MMP13 activity, but 100 and 200 μM of 444283 caused approximately a 60% reduction in MMP13 activity compared with saline.

MMP13 involved in several pathways and played different roles in them. We selected most pathways MMP13 participated on our site, such as AGE/RAGE pathway, Activation of Matrix Metalloproteinases, Assembly of collagen fibrils and other multimeric structures, which may be useful for your reference. Also, other proteins which involved in the same pathway with MMP13 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
AGE/RAGE pathwayLGALS3;AGER;MMP13;DIAPH1;JAK2;EGFR;TIRAP;MAPK14;JUN
Activation of Matrix MetalloproteinasesTIMP2B;CMA1;CTSLL;MMP15;MMP10;LOC100686744;MGC174857;FURINA;MGC174152
Assembly of collagen fibrils and other multimeric structuresCOL1A2;CTSL2;COL10A1A;COL4A5;COL5A3A;BMP1B;COL4A6;COL6A1;CTSS
Collagen degradationCOL23A1;COL13A1;ADAM9;MMP11;ADAM10;MMP20;MMP19;TLDC2;MGC174152
Collagen formationPCOLCE2;COL5A2;COL28A1D;BMP1;COL17A1A;COL1A2;COL3A1;MMP20;CTSB
Degradation of the extracellular matrixMMP8;CTRB1;COL13A1;ADAMTS1;MGC174152;TIMP2A;ADAM8A;EMID2;COL23A1
Endochondral OssificationPTHLHA;DDR2;PTH;SOX5;COL10A1A;KIF3A;COL10A1;SERPINH1;CDKN1C
Extracellular matrix organizationCOL2A1;BSG;TLL1;COL4A4;COL5A1;NID2;ADAM9;PTPRS;LOXL4

MMP13 has several biochemical functions, for example, calcium ion binding, calcium-dependent protein binding, collagen binding. Some of the functions are cooperated with other proteins, some of the functions could acted by MMP13 itself. We selected most functions MMP13 had, and list some proteins which have the same functions with MMP13. You can find most of the proteins on our site.

Function Related Protein
calcium ion bindingPCDH18A;CRACR2B;PPP3R2;PCDH2G3;PCDH2AB2;CDK5R1;VSNL1A;CDH7;F9B
calcium-dependent protein bindingMASP1;SYT8;SYT1;MASP2;TNNC1;SLC9A1;MYO1D;ANXA4;ALG2
collagen bindingSERPINH2;MTAP1A;PCOLCE;SPARC;DDR1;SMAD4;PODN;PCOLCE2;PDGFB
fibronectin bindingCCDC80;CTSK;IGFBP5B;MFAP2;THBS1;ITGAV;VEGFA;LACRT;LRRC15
low-density lipoprotein particle receptor bindingSACS;HSP90B1;SNX17;MESDC2;LRPAP1;AP2M1;APOE;DNAJA1;APOB
metalloendopeptidase activityADAM12;ADAMTS3;MMP1A;KEL;MMP20;MMP16;IDE;ADAMTS16;THOP1
zinc ion bindingESRRA;VPS8;EHMT2;ADAMTS15;PDLIM3B;DZIP3;APOBEC2;GATA2B;SETDB2

MMP13 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with MMP13 here. Most of them are supplied by our site. Hope this information will be useful for your research of MMP13.

Gene Family

MMPs

Research Area

Related articles

Ma, J; Zhou, D; et al. Keratocytes Create Stromal Spaces to Promote Corneal Neovascularization Via MMP13 Expression. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 55:-(2014).
Jackson, MT; Moradi, B; et al. Activation of Matrix Metalloproteinases 2, 9, and 13 by Activated Protein C in Human Osteoarthritic Cartilage Chondrocytes. ARTHRITIS & RHEUMATOLOGY 66:1525-1536(2014).
  • Q&As
  • Reviews

Q&As (5)

Ask a question
Can MMP13 be a potential therapeutic target for cancer treatment? 04/17/2023

Yes, targeting MMP13 has been explored as a potential therapeutic strategy to inhibit cancer metastasis and invasion.

Is MMP13 involved in cardiovascular diseases? 05/29/2022

Yes, MMP13 has been implicated in cardiovascular diseases by contributing to the remodeling of blood vessels and cardiac tissues.

How does MMP13 contribute to cancer progression? 11/18/2021

MMP13 facilitates tumor invasion and metastasis by breaking down the extracellular matrix and promoting the migration of cancer cells.

How is genetic variation in MMP13 associated with disease susceptibility? 03/03/2019

Certain genetic variations in the MMP13 gene have been linked to increased susceptibility to diseases such as osteoarthritis and cancer.

Are there any clinical trials targeting MMP13? 08/30/2017

Some clinical trials are investigating drugs that specifically target MMP13 in various cancer types to assess their efficacy and safety.

Customer Reviews (3)

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Reviews
12/05/2022

    Its reliability and versatility make it an essential tool for investigating MMP13 protein's expression, function, and structural characteristics.

    07/27/2020

      The use of MMP13 protein in this technique allows researchers to explore the intricate three-dimensional structure of the protein at a molecular level.

      10/13/2016

        MMP13 protein is highly recommended for researchers due to its excellent performance in WB experiments and its utility in protein electron microscopy structure analysis.

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