Recombinant Human MMP13 293 Cell Lysate
Cat.No. : | MMP13-4280HCL |
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Description : | Antigen standard for matrix metallopeptidase 13 (collagenase 3) (MMP13) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Tag : | Non |
Gene Name : | MMP13 matrix metallopeptidase 13 (collagenase 3) [ Homo sapiens ] |
Official Symbol : | MMP13 |
Synonyms : | MMP13; matrix metallopeptidase 13 (collagenase 3); matrix metalloproteinase 13 (collagenase 3); collagenase 3; CLG3; MMP-13; MANDP1; |
Gene ID : | 4322 |
mRNA Refseq : | NM_002427 |
Protein Refseq : | NP_002418 |
MIM : | 600108 |
UniProt ID : | P45452 |
Chromosome Location : | 11q22.3 |
Pathway : | Activation of Matrix Metalloproteinases, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem; Endochondral Ossification, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; Matrix Metalloproteinases, organism-specific biosystem; |
Function : | calcium ion binding; calcium-dependent protein binding; fibronectin binding; low-density lipoprotein particle receptor binding; metalloendopeptidase activity; peptidase activity; zinc ion binding; |
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Customer Reviews (3)
Write a reviewIts reliability and versatility make it an essential tool for investigating MMP13 protein's expression, function, and structural characteristics.
The use of MMP13 protein in this technique allows researchers to explore the intricate three-dimensional structure of the protein at a molecular level.
MMP13 protein is highly recommended for researchers due to its excellent performance in WB experiments and its utility in protein electron microscopy structure analysis.
Q&As (5)
Ask a questionYes, targeting MMP13 has been explored as a potential therapeutic strategy to inhibit cancer metastasis and invasion.
Yes, MMP13 has been implicated in cardiovascular diseases by contributing to the remodeling of blood vessels and cardiac tissues.
MMP13 facilitates tumor invasion and metastasis by breaking down the extracellular matrix and promoting the migration of cancer cells.
Certain genetic variations in the MMP13 gene have been linked to increased susceptibility to diseases such as osteoarthritis and cancer.
Some clinical trials are investigating drugs that specifically target MMP13 in various cancer types to assess their efficacy and safety.
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