Active Recombinant Human MMP13 protein, His-tagged

• Cat.No.: MMP13-2448H
• Product Overview: Active Recombinant Human MMP13 protein(P45452)(290~468 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 290-468 aa
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: Matrix Metalloproteinase 13 (MMP13) is a member of the matrix metalloproteinase(MMP) family. MMP13 has been proposed to participate in aggrecan degradationassociated with osteoarthritis and cleavage of type II collagen in osteoarthriticcartilage explants and in tumor progression and metastasis. In addition, it cancleave type I, III, IV, IX, X and XIV collagens and fibronectin. MMP13 is likely toplay a crucial role in the modulation of extracellular matrix degradation and cell-matrix interactions. Although gelatin zymography is mainly usedfor the detection of the MMP2 and MMP9, it also can be used for MMP13 dection. Briefly, various concentrations of MMP13 (1000ng, 500ng, 250ng, 125ng) weredenaturedby SDS loading buffer, electrophoresed through sodiumdodecylsulphat-polyacrylamide gel (SDS-PAGE; 10% gels) containing gelatin (1mg/mL) withnonreducing conditions. After renaturation, incubation and CCB-stained, active MMP13 would hydrolyze gelatin nearby, which was indicated by the whitebandson the gel. In this experiment we use heat-denatured MMP13 protein as negative control, and blood sample as positive control.
• Molecular Mass: 20.9kDa
• Purity: > 95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: MMP13 matrix metallopeptidase 13 (collagenase 3) [ Homo sapiens ]
• Official Symbol: MMP13
• Synonyms: MMP13; matrix metallopeptidase 13 (collagenase 3); matrix metalloproteinase 13 (collagenase 3); collagenase 3; CLG3; MMP-13; MANDP1
• Gene ID: 4322
• mRNA Refseq: NM_002427
• Protein Refseq: NP_002418
• MIM: 600108
• UniProt ID: P45452