Active Recombinant Rat Mmp13 protein, His-tagged

• Cat.No.: Mmp13-506R
• Product Overview: Active Recombinant Rat Mmp13 protein(P23097)(14~466 aa), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 14-466 aa
• Form: Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: Matrix Metalloproteinase 13 (MMP13) is a member of the matrix metalloproteinase (MMP) family. MMP13 has been proposed to participate in aggrecan degradation associated with osteoarthritis and cleavage of type II collagen in osteoarthritic cartilage explants and in tumor progression and metastasis. In addition, it can cleave type I, III, IV, IX, X and XIV collagens and fibronectin. MMP13 is likely to play a crucial role in the modulation of extracellular matrix degradation and cell-matrix interactions. Although gelatin zymography is mainly used for the detection of the MMP2 and MMP9, it also can be used for MMP13 dection. Briefly, various concentrations of MMP13 (1000ng, 500ng, 250ng, 125ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphat- polyacrylamide gel (SDS-PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP13 would hydrolyze gelatin nearby, which was indicated by the white bands on the gel. In this experiment we use heat-denatured MMP13 protein as negative control, and blood sample as positive control.
• Molecular Mass: 53.4kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 97% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Gene Information

• Gene Name: Mmp13 matrix metallopeptidase 13 [ Rattus norvegicus ]
• Official Symbol: Mmp13
• Synonyms: MMP13; matrix metallopeptidase 13; collagenase 3; MMP-13; UMRCASE; collagenase-3; matrix metalloproteinase 13; matrix metalloproteinase-13
• Gene ID: 171052
• mRNA Refseq: NM_133530
• Protein Refseq: NP_598214