Active Recombinant Human MMP13 (F175D) Protein

• Cat.No.: MMP13-01H
• Product Overview: Recombinant Human MMP13 Protein (residues 104-268, F175D mutant) without tag was expressed in E. coli.

Specification

• Description: This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11.
• Source: E. coli
• Species: Human
• Bio-activity: > 40 U/μg. Activity described as U=100 pmol/min at 25 centigrade using a colorimetric assay with thiopeptide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate.
• Molecular Mass: 18.6 kDa
• Protein length: 104-268
• AA Sequence: YNVFPRTLKWSKMNLTYRIVNYTPD MTHSEVEKAFKKAFKVWSDVTPLNF TRLHDGIADIMISFGIKEHGDDYPF DGPSGLLAHAFPPGPNYGGDAHFDD DETWTSSSKGYNLFLVAAHEFGHSL GLDHSKDPGALMFPIYTYTGKSHFM LPDDDVQGIQSLYGP
• Purity: > 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight of about 18.4 kDa.
• Usage: Enzyme kinetic studies, cleavage of target substrates and screening of inhibitors.
• Storage: -80 centigrade. After initial defrost, aliquot the product into individual tubes and refreeze at -80 centigrade. Avoid repeated freeze/thaw cycles.
• Concentration: 0.2 mg/mL
  The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 29910 M-1cm-1 calculated).
• Storage Buffer: Tris 20 mM pH 7.2, CaCl2 10 mM, ZnCl2 0.1 mM, NaCl 0.3 M, acetohydroxamic acid (AHA) 0.5 M.

Gene Information

• Gene Name: MMP13 matrix metallopeptidase 13 [ Homo sapiens (human) ]
• Official Symbol: MMP13
• Synonyms: MMP13; matrix metallopeptidase 13; CLG3; MDST; MANDP1; MMP-13; collagenase 3; matrix metalloproteinase 13 (collagenase 3); EC 3.4.24.-
• Gene ID: 4322
• mRNA Refseq: NM_002427
• Protein Refseq: NP_002418
• MIM: 600108
• UniProt ID: P45452

Reviews

12/05/2022

Its reliability and versatility make it an essential tool for investigating MMP13 protein's expression, function, and structural characteristics.

07/27/2020

The use of MMP13 protein in this technique allows researchers to explore the intricate three-dimensional structure of the protein at a molecular level.

10/13/2016

MMP13 protein is highly recommended for researchers due to its excellent performance in WB experiments and its utility in protein electron microscopy structure analysis.

Q&As

Can MMP13 be a potential therapeutic target for cancer treatment?

Yes, targeting MMP13 has been explored as a potential therapeutic strategy to inhibit cancer metastasis and invasion.

Is MMP13 involved in cardiovascular diseases?

Yes, MMP13 has been implicated in cardiovascular diseases by contributing to the remodeling of blood vessels and cardiac tissues.

How does MMP13 contribute to cancer progression?

MMP13 facilitates tumor invasion and metastasis by breaking down the extracellular matrix and promoting the migration of cancer cells.

How is genetic variation in MMP13 associated with disease susceptibility?

Certain genetic variations in the MMP13 gene have been linked to increased susceptibility to diseases such as osteoarthritis and cancer.

Are there any clinical trials targeting MMP13?

Some clinical trials are investigating drugs that specifically target MMP13 in various cancer types to assess their efficacy and safety.