NANOG

  • Official Full Name

    Nanog homeobox

  • Overview

    NANOG (Homeobox protein NANOG) is a member of the Nanog homeobox family of DNA-binding proteins. It is expressed in embryonic stem cells and confers pluripotency on these cells. Once embryonic stem cells become differentiated, NANOG expression is suppressed. NANOG is involved in the Hedgehog/Gli1 signaling pathway which has been implicated in the development and growth of various types of tumors.
  • Synonyms

    NANOG; Nanog homeobox; homeobox protein NANOG; ES cell-associated protein 4; homeobox transcription factor Nanog; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; ENK; ecat4; 2410002E02Rik;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Assay Kits
  • Chicken
  • Cynomolgus Monkey
  • Human
  • Mouse
  • Zebrafish
  • E.coli
  • HEK293
  • Mammalian Cell
  • Yeast
  • 13
  • TAT
  • Arg
  • His
  • His (Fc)
  • Avi
  • Myc
  • His|GST
  • SUMO
  • N/A
  • N
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human NANOG-130H Active Recombinant Human NANOG protein, Arginine-tagged E.coli Arg
Human NANOG-3604H Recombinant Human Nanog E.coli N/A
Human NANOG-3605H Recombinant Human NANOG Protein, His/GST-tagged E.coli His/GST Met1~Val305
Human NANOG-159H Recombinant Human NANOG Protein, 13-residue TAT-tagged E.coli 13-TAT
Human NANOG-3603H Recombinant Human NANOG, His-tagged E.coli His
Human NANOG-864H Recombinant Human NANOG Mammalian Cell His
Human NANOG-8468H Recombinant Human NANOG, His-tagged E.coli His
Human NANOG-3981HCL Recombinant Human NANOG 293 Cell Lysate HEK293 N/A
Human NANOG-4924H Recombinant Human NANOG protein, His&Myc-tagged E.coli His&Myc 1-305aa
Human NANOG-5824H Recombinant Human NANOG protein, His-tagged Yeast His 1-305aa
Human NANOG-3580H Recombinant Human NANOG Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Human NANOG-4923H Recombinant Human NANOG protein, His-SUMO-tagged E.coli His-SUMO 1-305aa
Human NANOG-144H Recombinant Human NANOG Protein, His-tagged E.coli His
Human NANOG-14H Recombinant Human NANOG Protein E.coli
Human NANOG-3580H-B Recombinant Human NANOG Protein Pre-coupled Magnetic Beads HEK293
Human NANOG-5188H Recombinant Human NANOG Protein (Trp153-Val305), N-His tagged E.coli N-His Trp153-Val305
Mouse Nanog-151M Recombinant Mouse Nanog, TAT-tagged E.coli TAT
Cynomolgus Monkey NANOG-725C Recombinant Cynomolgus NANOG Protein, His-tagged Mammalian Cell His
Cynomolgus Monkey NANOG-470C Recombinant Cynomolgus Monkey NANOG Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Cynomolgus Monkey NANOG-470C-B Recombinant Cynomolgus Monkey NANOG Protein Pre-coupled Magnetic Beads HEK293
Zebrafish NANOG-5682Z Recombinant Zebrafish NANOG Mammalian Cell His
Chicken NANOG-3937C Recombinant Chicken NANOG Mammalian Cell His
Kit-2296 Nanog (Human) Transcription Factor Activity Assay Kit N/A
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource

What is NANOG protein?

NANOG (Nanog homeobox) gene is a protein coding gene which situated on the short arm of chromosome 12 at locus 12p13. NANOG (Homeobox protein NANOG) is a member of the Nanog homeobox family of DNA-binding proteins. It is expressed in embryonic stem cells and confers pluripotency on these cells. Once embryonic stem cells become differentiated, NANOG expression is suppressed. NANOG is involved in the Hedgehog/Gli1 signaling pathway which has been implicated in the development and growth of various types of tumors. The NANOG protein is consisted of 305 amino acids and its molecular mass is approximately 34.6 kDa.

What is the function of NANOG protein?

NANOG acts as a transcriptional activator or repressor involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. And NANOG can also block bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes.

NANOG Related Signaling Pathway

By interacting with multiple signaling pathways, such as Wnt/β-catenin signaling pathway, TGF-β/BMP signaling pathway, Hippo/YAP signaling pathway, Oct4/Sox2 signaling pathway, etc., NANOG is involved in multiple biological processes such as self-renewal, pluripotency maintenance and cell fate determination of stem cells.

NANOG Related Diseases

NANOG protein is an important transcription factor associated with the maintenance of self-renewal and pluripotency of embryonic stem cells. Nanog protein is abnormally expressed in some tumors, including gastric cancer, lung cancer, breast cancer, ovarian cancer and colorectal cancer. Abnormal expression of NANOG may lead to impaired function of germ cells, resulting in infertility, or affect embryonic development and stem cell diseases.

NANOG-9.jpg

Fig1. Paradoxical effects of EVs from Nanog-overexpressing cancer cells on their metastasis. (Mikako Saito, 2022)

Bioapplications of NANOG

NANOG is a major marker of stem cells, especially embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). The self-renewal ability and pluripotency of stem cells can be assessed by detecting the expression level of NANOG protein. By regulating the expression of Nanog protein, the differentiation and development of stem cells can be promoted, which can be used to treat certain diseases.

Case study 1: Hee Yun, 2022

NANOG engages with tumour initiation and metastasis by regulating the epithelial-mesenchymal transition (EMT) in epithelial ovarian cancer (EOC). However, its role in association with pAMPKα, and its clinical significance in EOC have not been elucidated even though AMPK is known to degrade NANOG in various human cancers. Hence, this team investigated the role of pAMPKα and its association with NANOG as potential prognostic biomarkers in EOC.

Both NANOG and pAMPKα expression were significantly overexpressed in EOCs comparing nonadjacent normal epithelial tissues, benign tissues, and borderline tumours. In ovarian cancer cell lines, siRNA-mediated NANOG knockdown diminished migration and invasion properties by regulating the EMT process via the AMPK/mTOR signalling pathway.

NANOG-3.jpg

Fig1. Protein expression of NANOG and α-actinin was analysed by western blot (numbers below each blot are densitometric values).

NANOG-4.jpg
Fig2. Inhibition of the AMPK signalling pathway reverses the effect of NANOG knockdown on EMT.

Case study 2: Kyoung-Jae Choi, 2022

Human NANOG expression resets stem cells to ground-state pluripotency. Here this team identified the unique features of human NANOG that relate to its dose-sensitive function as a master transcription factor.

Full-length NANOG readily forms higher-order oligomers at low nanomolar concentrations, orders of magnitude lower than typical amyloids. Using single-molecule Förster resonance energy transfer and fluorescence cross-correlation techniques, the researchers show that NANOG oligomerization is essential for bridging DNA elements in vitro. Using chromatin immunoprecipitation sequencing and Hi-C 3.0 in cells, we validate that NANOG prion-like domain assembly is essential for specific DNA recognition and distant chromatin interactions. NANOG's unique ability to form prion-like assemblies could provide a cooperative and concerted DNA bridging mechanism that is essential for chromatin reorganization and dose-sensitive activation of ground-state pluripotency.

NANOG-5.jpg

Fig3. Autocorrelation FCS curves of (i) h6g-eGFP and GFP-tagged NANOG WT and W8A constructs (~10 nM) in HEK 293T mammalian cell lysates.

NANOG-6.jpg
Fig4. smFRET of ~100 pM each of Gata6-AF488 and Gata6-AF647 intermolecular diffusion in (i) the absence or (ii,iii) the presence of 250 nM NANOG W8A or WT NANOG.
NANOG-8.jpg

Fig2. Hypothetical model that WNT-mediated β-CATENIN and NANOG transactivate EZH2. (Chad Nakagawa, 2023)

NANOG involved in several pathways and played different roles in them. We selected most pathways NANOG participated on our site, such as Signaling pathways regulating pluripotency of stem cells, Proteoglycans in cancer, which may be useful for your reference. Also, other proteins which involved in the same pathway with NANOG were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Signaling pathways regulating pluripotency of stem cellsINHBC;FZD9;LIF;BMI1;DLX5;BMP4;MYF5;MAP2K2;WNT11
Proteoglycans in cancerPTPN11;TFAP4;PDPK1;SLC9A1;PIK3R1;FZD3;HSPB2;PRKCB;WNT9A

NANOG has several biochemical functions, for example, DNA binding, sequence-specific DNA binding, transcription corepressor activity. Some of the functions are cooperated with other proteins, some of the functions could acted by NANOG itself. We selected most functions NANOG had, and list some proteins which have the same functions with NANOG. You can find most of the proteins on our site.

Function Related Protein
DNA bindingDEM1;TRIM21;ZNF521;CLOCKA;ZNF180;ZNF134;ZSCAN18;ZNF285A;TCEA2
sequence-specific DNA bindingMEF2A;LBX1B;NR2F6A;TA;ENG1A;NR6A1;NFYBA;FOXA3;HOXC11A
transcription corepressor activityMAP3K10;CDYL;ENO1;CCAR1;HDAC4;PEX14;CRYM;ZMYND11;PBXIP1
transcription factor activity, sequence-specific DNA bindingZNF187;NPAS1;FUBP1;FOSL1;FOXH1;HMGB2;SMAD3B;NR1D4A;SIX4

NANOG has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with NANOG here. Most of them are supplied by our site. Hope this information will be useful for your research of NANOG.

Research Area

Related articles

Khosravi-Farsani, S; Amidi, F; et al. Isolation and Enrichment of Mouse Female Germ Line Stem Cells. CELL JOURNAL 16:406-415(2015).
Tyagi, D; Perez, JB; et al. Detection of embryonic stem cell lysate biomarkers by surface plasmon resonance with reduced nonspecific adsorption. ANALYTICAL BIOCHEMISTRY 471:29-37(2015).
  • Q&As
  • Reviews

Q&As (7)

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Are there any disease associations linked to NANOG dysregulation? 08/04/2021

Dysregulation of NANOG expression has been implicated in various diseases, including cancer and developmental disorders. Aberrant NANOG expression can promote tumor initiation, progression, and metastasis, as well as contribute to embryonic developmental abnormalities.

How does NANOG contribute to immune regulation and tolerance? 07/25/2020

NANOG has been shown to play a role in immune regulation and tolerance by promoting the generation of regulatory T cells (Tregs) and inhibiting the differentiation of effector T cells. It modulates the expression of genes involved in Treg development and function, thereby contributing to immune homeostasis.

What are the regulatory mechanisms controlling NANOG expression? 07/19/2020

NANOG expression is regulated by a complex network of transcription factors, epigenetic modifications, and signaling pathways. Transcription factors like OCT4, SOX2, and KLF4 bind to the NANOG promoter region, while epigenetic modifications such as DNA methylation and histone acetylation also influence its expression.

What is the cellular localization of NANOG protein in the body? 02/21/2020

NANOG protein is predominantly localized in the nucleus of embryonic stem cells (ESCs) and in the inner cell mass of preimplantation embryos. Its nuclear localization is essential for its role in maintaining pluripotency and self-renewal in ESCs.

What potential therapeutic implications does NANOG hold? 02/13/2018

Understanding the role of NANOG in pluripotency and disease pathogenesis opens up potential therapeutic avenues. Targeting NANOG and its associated signaling pathways may provide strategies for regenerative medicine, cancer therapy, and the treatment of developmental disorders. Further research is needed to fully explore the therapeutic potential of NANOG modulation.

How is the expression of NANOG regulated during embryonic development and cellular differentiation? 12/01/2017

The expression of NANOG is tightly controlled during embryonic development and cellular differentiation. Various signaling pathways, including the Wnt, BMP, and FGF pathways, are involved in regulating NANOG expression. Additionally, epigenetic modifications, such as DNA methylation and histone modifications, can also influence NANOG expression. During cellular differentiation, NANOG expression is generally downregulated as cells acquire specialized identities. Transcription factors and microRNAs also play important roles in regulating NANOG expression and mediating its temporal and spatial expression patterns.

What are the signaling pathways regulated by NANOG? 02/14/2017

NANOG regulates several signaling pathways involved in pluripotency, including the Wnt/β-catenin, TGF-β/Smad, and PI3K/AKT pathways. It interacts with key components of these pathways, modulating their activity to maintain the pluripotent state of stem cells.

Customer Reviews (3)

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Reviews
03/31/2022

    High efficiency characterizes the use of this experimental reagent, rendering my research work seamless and productive.

    10/03/2018

      The operation of the reagent is straightforward, without the need for additional equipment or materials.

      04/13/2018

        High quality and stability ensure the accuracy and reliability of my experimental results while using this reagent.

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